首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪白介素8(IL-8/CXCL8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CXCL8含量。CXCL8，即CXC趋化因子配体8，也称为白细胞介素-8，是一种重要的趋化因子，主要由免疫细胞和肿瘤细胞分泌。它通过激活中性粒细胞和其他免疫细胞，参与炎症反应和肿瘤的发生发展。CXCL8的研究机制涉及与受体CXCR1和CXCR2的结合，以及下游信号通路的激活，如MAPK和PI3K/Akt通路。试剂盒检测范围为125 pg/mL-8000 pg/mL，灵敏度为31.25 pg/mL。适用于体外细胞因子分泌分析、感染或炎症模型中免疫应答评估、疫苗研发过程中的免疫效果监测等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; Alveolar macrophage chemotactic factor I ELISA Kit; AMCF-I ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
缩写：CXCL8
Uniprot No.：P26894
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-97 | |||||
回收率：
| The recovery of pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-104 | ||||
| EDTA plasma (n=4) | 85 | 81-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 80002.6622.6012.6322.460 40002.2152.1612.1882.016 20001.4981.4041.4511.279 10000.8710.8190.8450.673 5000.5100.5070.5090.337 2500.3800.3590.3700.198 1250.2710.2610.2660.094 00.1740.1700.172 | ||||||
货期：3-5 working days
×
