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产品描述：犬白介素8(IL-8/CXCL8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CXCL8含量。CXCL8作为一个靶点，它属于趋化因子家族，与炎症反应、细胞迁移等密切相关。研究发现，它可以通过与特定受体结合，激活下游信号通路，在肿瘤、自身免疫病等疾病发展进程中发挥作用，是当前药物研发等领域的重要研究对象。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.146 ng/mL。本试剂盒通过高灵敏度抗体组合实现0.312 ng/mL至20 ng/mL的宽泛检测范围，可精准量化低丰度目标蛋白，满足科研场景中细胞因子动态监测、炎症模型评估或药物干预效果分析等需求。其操作流程兼容常规实验室设备，适用于兽医基础研究、实验动物模型开发及生物制剂研发等领域，为探索犬类免疫系统功能及相关疾病机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
缩写：CXCL8
Uniprot No.：P41324
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.146 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of dog IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-94 | ||||
| EDTA plasma (n=4) | 88 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 203.2213.2953.2583.157 102.5232.7112.6172.516 51.7911.9301.8611.760 2.51.0351.0451.0400.939 1.250.4660.4850.4760.375 0.6250.2490.2590.2540.153 0.3120.1570.1670.1620.061 00.1030.0990.101 | ||||||
货期：3-5 working days
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