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产品描述：豚鼠免疫球蛋白G(IgG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgG含量。IgG是人体免疫系统中的主要抗体，由B细胞产生，具有抗菌、抗病毒和中和毒素等功能。研究机制涉及B细胞分化为浆细胞，分泌IgG抗体，以及抗体与抗原结合后的免疫反应。近年来，研究发现肿瘤细胞也能合成IgG，称为肿瘤来源免疫球蛋白（cancer-IgG），其表达与肿瘤的侵袭和转移密切相关。试剂盒检测范围为62.5 μg/mL-1000 μg/mL，灵敏度为62.5 μg/mL。该产品适用于科研领域，如感染性疾病模型中免疫应答动态监测、疫苗研发中抗体效价评估，或基础免疫学研究中豚鼠体液免疫功能分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgG
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：62.5 μg/mL-1000 μg/mL
灵敏度：62.5 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 99 | ||||
| Range % | 95-103 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:80 | Average % | 92 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of guinea pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-104 | ||||
| EDTA plasma (n=4) | 96 | 84-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 10000.1390.1410.140 5000.3000.3120.306 2500.6000.6040.602 1250.8690.8720.871 62.51.3721.3951.384 02.9162.7092.813 | ||||||
货期：3-5 working days
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