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Human Insulin-like growth factor-binding protein 6(IGFBP6) ELISA kit

  • 中文名： 人胰岛素样生长因子结合蛋白6(IGFBP6)酶联免疫试剂盒
货号: IPD-EL011100HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人胰岛素样生长因子结合蛋白6(IGFBP6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的IGFBP6含量。IGFBP6是一种重要靶点。它能与胰岛素样生长因子结合，尤其对IGF-II有高亲和力。其背景方面涉及细胞生长、增殖等生理过程。研究机制主要围绕其对IGF-II活性的抑制，以此影响肿瘤细胞的生长、迁移等，在肿瘤等疾病研究中有潜在价值。试剂盒检测范围为0.78 ng/mL-50 ng/mL，灵敏度为0.195 ng/mL。该产品适用于体外研究IGFBP6在肿瘤微环境调控、代谢紊乱机制、细胞增殖与凋亡等相关领域的表达变化，为探索IGF信号通路在疾病发生发展中的作用提供可靠工具，尤其适用于需要高灵敏度检测微量样本的科研场景，如临床前模型的组织分析或体液生物标志物研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    IBP 6 ELISA Kit; IBP-6 ELISA Kit; IBP6 ELISA Kit; IBP6_HUMAN ELISA Kit; IGF binding protein 6 ELISA Kit; IGF-binding protein 6 ELISA Kit; IGFBP 6 ELISA Kit; IGFBP-6 ELISA Kit; IGFBP6 ELISA Kit; Insulin like growth factor binding protein 6 ELISA Kit; Insulin-like growth factor-binding protein 6 ELISA Kit
  • 缩写：
    IGFBP6
  • Uniprot No.：
    P24592
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma,tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)6.1686.3346.3066.2726.3596.242SD0.0350.0380.0420.0480.0540.051CV(%)3.4033.6254.0194.6115.1364.916







  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)



    1:5

    Average %

    96



    Range %

    92-103



    1:10

    Average %

    90



    Range %

    86-98



    1:20

    Average %

    93



    Range %

    89-99



    1:40

    Average %

    91



    Range %

    85-98










  • 回收率：

    The recovery of human IGFBP6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%



    Serum (n=5)

    95

    89-102



    EDTA plasma (n=4)

    92

    88-99










  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


    ng/ml

    OD1

    OD2

    Average

    Corrected



    50

    3.109

    3.089

    3.099

    2.949



    25

    2.418

    2.425

    2.421

    2.271



    12.5

    1.645

    1.665

    1.655

    1.505



    6.25

    1.056

    1.034

    1.045

    0.895



    3.13

    0.666

    0.635

    0.650

    0.500



    1.56

    0.379

    0.363

    0.371

    0.221



    0.78

    0.277

    0.262

    0.269

    0.119



    0

    0.154

    0.147

    0.150












  • 货期：
    3-5 working days

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