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产品描述:人胰岛素样生长因子结合蛋白3(IGFBP-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的IGFBP3含量。IGFBP3(胰岛素样生长因子结合蛋白-3)是一种在人体中编码的蛋白质,主要功能是延长IGF-1和IGF-2的半衰期,并阻止它们与细胞表面受体的相互作用。IGFBP3在调节IGF信号通路中发挥重要作用,其表达水平与多种疾病,包括癌症的发生和发展密切相关。研究IGFBP3的机制有助于理解其在疾病过程中的作用,并为开发新的治疗策略提供依据。试剂盒检测范围为0.78 ng/mL-50 ng/mL,灵敏度为0.195 ng/mL。本产品适用于基础医学研究,如探究 IGF 信号通路在代谢调控、肿瘤微环境或组织修复中的作用机制,支持科研人员对血液样本或组织提取物中 IGFBP - 3 的动态变化进行系统性分析。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Acid stable subunit of the 140 K IGF complex ELISA Kit; Binding protein 29 ELISA Kit; Binding protein 53 ELISA Kit; BP 53 ELISA Kit; BP53 ELISA Kit; Growth hormone dependent binding protein ELISA Kit; IBP 3 ELISA Kit; IBP-3 ELISA Kit; IBP3 ELISA Kit; IBP3_HUMAN ELISA Kit; IGF binding protein 3 ELISA Kit; IGF-binding protein 3 ELISA Kit; IGFBP 3 ELISA Kit; IGFBP-3 ELISA Kit; IGFBP3 ELISA Kit; Insulin Like Growth Factor Binding Protein 3 ELISA Kit; Insulin-like growth factor binding protein 3 precursor ELISA Kit; Insulin-like growth factor-binding protein 3 ELISA Kit
缩写:IGFBP3
Uniprot No.:P17936
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.78 ng/mL-50 ng/mL
灵敏度:0.195 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:10 | Average % | 92 | ||||
Range % | 82-103 | |||||
1:20 | Average % | 95 | ||||
Range % | 91-99 | |||||
1:40 | Average % | 95 | ||||
Range % | 90-100 | |||||
1:80 | Average % | 91 | ||||
Range % | 85-100 |
回收率:
The recovery of human IGFBP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 98 | 92-106 | ||||
EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected 502.1192.1192.1191.957 251.5601.5211.5411.379 12.51.0121.0801.0460.884 6.250.7160.7260.7210.559 3.120.4710.4540.4630.301 1.560.3210.3340.3280.166 0.780.2350.2430.2390.077 00.1610.1630.162 |
货期:3-5 working days
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