首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒

Rat Insulin-like growth factor 1,IGF-1 ELISA Kit

  • 中文名： 大鼠胰岛素样生长因子1(IGF-1)ELISA Kit
货号: IPD-E04582r
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    大鼠胰岛素样生长因子1(IGF-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IGF1含量。IGF1即胰岛素样生长因子 1，在人体生长发育、细胞增殖分化等生理过程中发挥重要作用。研究发现，它与肿瘤发生发展、代谢性疾病等密切相关。其研究机制主要围绕信号通路展开，探索阻断或调控相关信号以治疗疾病的可能。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.151 ng/mL。可广泛应用于大鼠模型的生长发育研究、代谢疾病机制探索、细胞培养体系中IGF - 1分泌水平监测，以及组织特异性表达的定量分析，为动物实验和体外研究提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Igf1 ELISA Kit; Igf-1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
  • 缩写：
    IGF1
  • Uniprot No.：
    P08025
  • 种属：
    Rattus norvegicus (Rat)
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.


  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



  • 本试剂盒所含材料：



    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IGF-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled IGF-1 antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.

    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days


      客户数据及评论

      折叠内容

      大包装询价

      ×