首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人绒毛膜促性腺激素(hCG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液样本中的hCG含量。hCG（人绒毛膜促性腺激素）是一种由胎盘分泌的激素，主要功能是刺激黄体，维持妊娠。它在受精卵着床后不久开始产生，并通过血液和尿液检测来判断妊娠。hCG水平在怀孕初期迅速上升，并在整个妊娠期间维持在一定水平。研究hCG有助于了解妊娠进程，并在辅助生殖技术中应用。试剂盒检测范围为8 mIU/mL-240 mIU/mL，灵敏度为2 mIU/mL。适用于妊娠相关机制研究、肿瘤标志物探索及生殖医学体外实验等领域，例如通过尿液样本分析激素代谢规律，或利用细胞培养上清液评估体外模型中hCG分泌动态。本试剂盒为生殖生物学、肿瘤学及发育医学等基础研究提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CGB3 ELISA Kit; CGB; ELISA Kit; CGB5; ELISA Kit; CGB8 ELISA Kit; Choriogonadotropin subunit beta 3 ELISA Kit; Choriogonadotropin subunit beta ELISA Kit; CG-beta ELISA Kit; Chorionic gonadotropin chain beta ELISA Kit
缩写：hCG
Uniprot No.：P0DN86
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, cell culture supernates
检测范围：8 mIU/mL-240 mIU/mL
灵敏度：2 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human HCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 2402.5402.5962.5682.532 1001.2791.2991.2891.253 400.6380.6320.6350.599 160.2850.2910.2880.252 80.1590.1550.1570.121 00.0360.0350.036 | ||||||
货期：3-5 working days
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