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产品描述：人肾损伤分子1(Kim-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆样本中的Kim-1含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.043 ng/mL。支持科研人员通过非侵入性样本（如尿液）或组织匀浆开展肾损伤相关研究，例如药物肾毒性评估、急性肾损伤模型动态监测或慢性肾病进展机制分析，为探索肾脏疾病的分子机制及潜在干预策略提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD365 ELISA Kit; HAVCR 1 ELISA Kit; HAVCR ELISA Kit; HAVcr-1 ELISA Kit; Havcr1 ELISA Kit; Hepatitis A virus cellular receptor 1 ELISA Kit; Kidney injury molecule 1 ELISA Kit; KIM 1 ELISA Kit; KIM-1 ELISA Kit; T cell immunoglobin domain and mucin domain protein 1 ELISA Kit; T cell immunoglobulin mucin family member 1 ELISA Kit; T cell immunoglobulin mucin receptor 1 ELISA Kit; T-cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit; T-cell membrane protein 1 ELISA Kit; TIM ELISA Kit; TIM-1 ELISA Kit; TIM1 ELISA Kit; TIMD 1 ELISA Kit; TIMD-1 ELISA Kit; TIMD1 ELISA Kit; TIMD1_HUMAN ELISA Kit
缩写：Kim-1
Uniprot No.：Q96D42
种属：Homo sapiens (Human)
样本类型：serum, urine, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.043 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-96 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 99-109 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 94-106 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of human Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 96-108 | ||||
| EDTA plasma (n=4) | 90 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.4092.5652.4872.317 101.5991.5181.5591.389 51.0121.0031.0080.838 2.50.5870.5780.5830.413 1.250.4330.4630.4480.278 0.6250.3020.3040.3030.133 0.3120.2310.2350.2330.063 00.1740.1650.170 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human Kim-1 antibody.
Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated Kim-1 antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic.
Substrate A (1 x 7 ml)
Substrate B (1 x 7 ml)
One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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