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Human Transmembrane glycoprotein NMB(GPNMB) ELISA kit

  • 中文名： 人跨膜糖蛋白NMB(GPNMB)酶联免疫试剂盒
货号: IPD-EL009727HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人跨膜糖蛋白NMB(GPNMB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPNMB含量。GPNMB是一个受到关注的靶点。它在多种生物学过程中发挥作用，其表达异常与一些疾病相关，如肿瘤。研究机制方面，主要聚焦于其在细胞增殖、迁移、侵袭等过程中的作用途径，以及探索针对它开发治疗药物，以干预疾病进展。试剂盒检测范围为0.78 ng/mL-50 ng/mL，灵敏度为0.195 ng/mL。支持基础研究中对GPNMB蛋白表达调控、疾病相关分子机制探索及药物干预效果评估等科研需求，例如黑色素瘤转移研究、骨关节炎模型分析或阿尔茨海默病相关神经炎症实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Glycoprotein (transmembrane) nmb ELISA Kit; Glycoprotein nmb ELISA Kit; Glycoprotein nmb like protein ELISA Kit; Gpnmb ELISA Kit; GPNMB_HUMAN ELISA Kit; HGFIN ELISA Kit; NMB ELISA Kit; Osteoactivin ELISA Kit; Transmembrane glycoprotein ELISA Kit; Transmembrane glycoprotein HGFIN ELISA Kit; Transmembrane glycoprotein NMB ELISA Kit
  • 缩写：
    GPNMB
  • Uniprot No.：
    Q14956
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human GPNMB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    104


    Range %

    97-111


    1:2

    Average %

    96


    Range %

    90-100


    1:4

    Average %

    100


    Range %

    95-105


    1:8

    Average %

    90


    Range %

    85-96


  • 回收率：

    The recovery of human GPNMB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    101

    96-107


    EDTA plasma (n=4)

    96

    90-103


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    50

    2.159

    2.234

    2.197

    2.015


    25

    1.697

    1.667

    1.682

    1.501


    12.5

    1.087

    1.098

    1.093

    0.912


    6.25

    0.730

    0.719

    0.724

    0.543


    3.12

    0.479

    0.491

    0.485

    0.304


    1.56

    0.345

    0.353

    0.349

    0.168


    0.78

    0.255

    0.244

    0.250

    0.069


    0

    0.177

    0.185

    0.181




  • 货期：
    3-5 working days

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