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Human follicle-stimulating hormone,FSH ELISA Kit

  • 中文名: 人促卵泡素(FSH)ELISA Kit
货号: IPD-E06867h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人促卵泡素(FSH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的FSH含量。促卵泡激素(FSH)是一种由垂体前叶分泌的糖蛋白激素,对性腺功能至关重要。它通过作用于性腺(卵巢和睾丸)的特定细胞,调节卵泡生长、雌激素产生、排卵和精子生成。FSH的活性形式是糖基化的异源二聚体,由α和β两条多肽组成,其作用机理涉及与靶细胞表面的G蛋白偶联受体结合,引发细胞内信号传导,从而调控相关生理过程。试剂盒检测范围为2 mIU/ml-50 mIU/ml.,灵敏度为1 mIU/ml.。适用于实验室开展生殖健康机制研究、生育力评估模型构建或激素相关疾病机理探索等科研项目;广泛用于基础医学、动物科学等领域中FSH表达水平的定量分析,为生殖系统功能调控研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    FSHBFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
  • 缩写:
    FSH
  • Uniprot No.:
    P01225
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    2 mIU/ml-50 mIU/ml.
  • 灵敏度:
    1 mIU/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    105


    Range %

    99-109


    1:2

    Average %

    98


    Range %

    95-102


    1:4

    Average %

    94


    Range %

    89-98


    1:8

    Average %

    101


    Range %

    98-107


  • 回收率:

    The recovery of human FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    91-99


    EDTA plasma (n=4)

    99

    94-108


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    mIU/ml

    OD1

    OD2

    Average

    Corrected


    50

    1.856

    1.793

    1.825

    1.734


    20

    0.899

    0.907

    0.903

    0.812


    10

    0.453

    0.427

    0.440

    0.349


    5

    0.256

    0.269

    0.263

    0.172


    2

    0.148

    0.116

    0.132

    0.041


    0

    0.089

    0.092

    0.091




  • 货期:
    3-5 working days

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