Mouse Fibronectin,FN ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04552m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠纤连蛋白(FN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FN1含量。FN1，即纤连蛋白1，是一种重要的细胞外基质蛋白，参与细胞粘附、迁移等生物过程。研究显示，FN1在骨骼疾病、代谢调节等方面发挥关键作用。近期研究发现，运动可通过促进骨骼肌分泌FN1，激活肝脏自噬，改善胰岛素敏感性，从而促进机体健康。试剂盒检测范围为2.7 ng/ml- 2000 ng/ml，灵敏度为2.7 ng/ml。基础研究中的细胞外基质功能探索、组织损伤修复模型评估或炎症相关疾病机制研究，为体外实验提供可靠检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Fn1Fibronectin ELISA kit; FN) [Cleaved into: Anastellin] ELISA kit
缩写：
FN1
Uniprot No.：
P11276
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
2.7 ng/ml - 2000 ng/ml
灵敏度：
2.7 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	97
	Range %	94-1.5
1:2	Average %	95
	Range %	91-105
1:4	Average %	101
	Range %	95-108
1:8	Average %	87
	Range %	80-99

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
2000	2.427	2.534	2.480	2.377
666	2.101	2.237	2.169	2.066
222	1.706	1.738	1.722	1.619
74	1.110	1.133	1.121	1.018
24.6	0.697	0.731	0.714	0.611
8.2	0.415	0.435	0.425	0.322
2.7	0.273	0.284	0.278	0.175
0	0.106	0.101	0.103