| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANXA11 |
| Uniprot No | P50995 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSYPGYPPPP GGYPPAAPGG GPWGGAAYPP PPSMPPIGLD NVATYAGQFN QDYLSGMAAN MSGTFGGANM PNLYPGAPGA GYPPVPPGGF GQPPSAQQPV PPYGMYPPPG GNPPSRMPSY PPYPGAPVPG QPMPPPGQQP PGAYPGQPPV TYPGQPPVPL PGQQQPVPSY PGYPGSGTVT PAVPPTQFGS RGTITDAPGF DPLRDAEVLR KAMKGFGTDE QAIIDCLGSR SNKQRQQILL SFKTAYGKDL IKDLKSELSG NFEKTILALM KTPVLFDIYE IKEAIKGVGT DEACLIEILA SRSNEHIREL NRAYKAEFKK TLEEAIRSDT SGHFQRLLIS LSQGNRDEST NVDMSLAQRD AQELYAAGEN RLGTDESKFN AVLCSRSRAH LVAVFNEYQR MTGRDIEKSI CREMSGDLEE GMLAVVKCLK NTPAFFAERL NKAMRGAGTK DRTLIRIMVS RSETDLLDIR SEYKRMYGKS LYHDISGDTS GDYRKILLKI CGGND |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANXA11重组蛋白的3篇示例参考文献，内容基于领域内已知研究方向综合模拟，供参考：
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1. **文献名称**：*"Recombinant ANXA11 protein suppresses hepatocellular carcinoma progression by modulating ERK signaling"*
**作者**：Zhang L, et al.
**摘要**：研究通过大肠杆菌系统表达并纯化ANXA11重组蛋白，发现其可通过抑制ERK磷酸化降低肝癌细胞侵袭性，提示其在癌症治疗中的潜在应用。
2. **文献名称**：*"Structural insights into ANXA11-calcium interactions: Implications for ALS pathogenesis"*
**作者**：Smith J, et al.
**摘要**：利用重组ANXA11蛋白进行晶体结构分析，揭示其与钙离子结合的特异性位点，为解释ANXA11突变导致C9orf72相关ALS的分子机制提供依据。
3. **文献名称**：*"ANXA11 regulates lysosomal trafficking via recombinant protein-based interaction assays"*
**作者**：Chen Y, et al.
**摘要**：通过体外互作实验证实重组ANXA11蛋白与动力蛋白(dynein)复合体结合，调控溶酶体运输功能，为细胞自噬异常相关疾病研究奠定基础。
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**注**：以上文献为模拟示例，实际研究中建议通过数据库(如PubMed、Web of Science)以关键词“ANXA11 recombinant protein”或“ANXA11 expression/purification”检索最新文献。ANXA11的研究热点多集中于神经退行性疾病(如ALS)和肿瘤调控通路。
**Background of ANXA11 Recombinant Protein**
ANXA11 (Annexin A11) is a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins implicated in diverse cellular processes, including membrane organization, vesicle trafficking, cell division, and apoptosis. Structurally, ANXA11 contains a conserved C-terminal annexin repeat domain that mediates calcium-dependent membrane interactions and a unique N-terminal region rich in low-complexity sequences, which may facilitate protein-protein interactions and phase separation. This distinctive architecture enables ANXA11 to participate in dynamic cellular activities, such as regulating calcium signaling, RNA transport, and mitotic spindle assembly.
The recombinant ANXA11 protein is typically produced using expression systems like *E. coli* or mammalian cell lines, ensuring proper post-translational modifications and functional integrity. It is often fused with tags (e.g., His-tag) for simplified purification and detection. Recombinant ANXA11 serves as a vital tool for studying its biochemical properties, interactome, and role in diseases. For instance, ANXA11 mutations or dysregulation have been linked to amyotrophic lateral sclerosis (ALS), where cytoplasmic mislocalization and aggregation of the protein disrupt RNA metabolism. In cancer, ANXA11 overexpression is associated with tumor progression in pancreatic, hepatocellular, and colorectal carcinomas, potentially influencing cell invasion and chemoresistance.
Research utilizing ANXA11 recombinant protein has clarified its interactions with partners like calcyclin (S100A6) and its involvement in calcium-dependent signaling pathways. Additionally, it aids in developing diagnostic assays and therapeutic strategies targeting ANXA11-associated pathologies. Its role as a potential biomarker or drug target underscores its relevance in translational medicine. Overall, ANXA11 recombinant protein is a critical resource for unraveling the molecular mechanisms of cellular regulation and disease pathogenesis.
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