首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠叶酸(FA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的FA含量。叶酸是一种水溶性B族维生素，对DNA合成、修复和甲基化至关重要。在大鼠研究中，叶酸缺乏会影响生长发育，可能通过调节胰岛素样生长因子（IGFs）系统的甲基化水平，进而影响胚胎发育和细胞增殖。叶酸还与DNA甲基化、细胞代谢和神经管缺陷等过程密切相关。试剂盒检测范围为19.5 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。本试剂盒适用于科研领域中对大鼠叶酸代谢相关研究，包括膳食营养评估、叶酸缺乏模型构建、药物干预效果分析等基础实验，为探索叶酸在生长发育、造血功能及神经系统中的作用机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：FA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：19.5 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat folic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 86-104 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 83-99 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 80-98 | |||||
回收率：
| The recovery of rat folic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 86-102 | ||||
| EDTA plasma (n=4) | 94 | 88-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 50000.3240.3260.325 12500.5140.5280.521 3120.8170.8140.816 781.2811.2931.287 19.51.6741.6351.655 01.9481.9661.957 | ||||||
货期：3-5 working days
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