Human Endostatin,ES ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E07973h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人内皮抑素(ES)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的ES含量。人内皮抑素（ES）是一种内源性抗血管生成蛋白，能特异性抑制血管内皮细胞增殖和迁移，从而抑制肿瘤血管生成。其作用机制包括调控内皮细胞表面蛋白表达、胞内信号通路，以及促进内皮细胞凋亡。研究显示，ES通过抑制VEGF介导的信号转导和bcl-2/bcl-XL、bad表达，阻断血管生成，抑制肿瘤生长和转移。试剂盒检测范围为15.6 pg/ml-1000 pg/ml，灵敏度为3.9 pg/ml。适用于肿瘤模型研究、血管生成相关机制探索、药物干预效果评估等科研领域，例如分析肿瘤组织或血液样本中 ES 表达水平与疾病进展的关联性，或评估抗血管生成治疗对 ES 动态变化的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
FLJ27325, FLJ34914, KNO, KNO1, MGC74745, alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP
缩写：
ES
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/ml -1000 pg/ml
灵敏度：
3.9 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:200	Average %	87
	Range %	80-94
1:400	Average %	93
	Range %	88-100
1:800	Average %	92
	Range %	86-100
1:1600	Average %	90
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	1.976	2.034	2.005	1.827
500	1.120	1.167	1.143	0.966
250	0.751	0.728	0.740	0.562
125	0.494	0.519	0.506	0.329
62.5	0.337	0.321	0.329	0.151
31.2	0.242	0.253	0.247	0.070
15.6	0.221	0.214	0.218	0.040
0	0.180	0.175	0.178