Mouse Epidermal growth factor,EGF ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08028m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠表皮生长因子(EGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EGF含量。EGF即表皮生长因子，广泛存在于人体组织和体液中。它能与细胞膜上的受体结合，激活下游信号通路，调控细胞生长、增殖和分化。在医学研究中，其信号通路异常与多种疾病相关，是药物研发等领域的重要靶点。试剂盒检测范围为7.8 pg/mL-500 pg/mL，灵敏度为1.95 pg/mL。主要应用于基础科研场景，包括但不限于小鼠疾病模型构建、药物干预效果评估、细胞培养上清液分析等实验体系，为探索EGF在生理调控或病理状态下的动态变化提供可靠工具，实验数据需结合其他分子生物学方法进行系统验证。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
EgfPro-epidermal growth factor ELISA Kit; EGF) [Cleaved into: Epidermal growth factor] ELISA Kit
缩写：
EGF
Uniprot No.：
P01132
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
7.8 pg/mL-500 pg/mL
灵敏度：
1.95 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Others
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:2	Average %	89
	Range %	80-92
1:4	Average %	93
	Range %	91-105
1:8	Average %	95
	Range %	90-103
1:16	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
500	2.638	2.684	2.661	2.503
250	2.154	2.067	2.111	1.953
125	1.671	1.638	1.655	1.497
62.5	1.126	1.079	1.103	0.945
31.2	0.693	0.711	0.702	0.544
15.6	0.382	0.363	0.373	0.215
7.8	0.295	0.284	0.290	0.132
0	0.161	0.155	0.158