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Human Epidermal growth factor,EGF ELISA Kit

  • 中文名： 人表皮生长因子(EGF)ELISA Kit
货号: IPD-E08027h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人表皮生长因子(EGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的EGF含量。EGF（表皮生长因子）是一种6-kDa的蛋白质，具有53个氨基酸残基和三个分子内二硫键。通过与细胞表面的同源受体EGFR结合，EGF能刺激细胞生长、分化和存活，影响组织器官的生长和分化。EGF的研究涉及其与EGFR的结合机制以及信号转导过程。试剂盒检测范围为7.8 pg/ml-500 pg/ml.，灵敏度为1.95 pg/mL。科研人员可将其应用于体外药物作用评估、疾病模型中的EGF动态监测、干细胞分化研究或再生医学相关实验，例如在皮肤修复研究中追踪EGF表达水平变化，或在肿瘤微环境研究中分析生长因子调控机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Beta urogastrone ELISA Kit; beta-urogastrone ELISA Kit; EGF ELISA Kit; EGF_HUMAN ELISA Kit; Epidermal growth factor ELISA Kit; HOMG4 ELISA Kit; OTTHUMP00000219721 ELISA Kit; OTTHUMP00000219722 ELISA Kit; Pro epidermal growth factor ELISA Kit; URG ELISA Kit; Urogastrone ELISA Kit
  • 缩写：
    EGF
  • Uniprot No.：
    P01133
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    7.8 pg/ml-500 pg/ml.
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)63.79965.35660.13562.31564.31564.315SD0.0160.0270.0150.0250.0270.020CV(%)4.5085.1014.4887.3737.9905.377
  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    102


    Range %

    98-105


    1:2

    Average %

    98


    Range %

    94-103


    1:4

    Average %

    96


    Range %

    90-101


    1:8

    Average %

    93


    Range %

    88-99









  • 回收率：

    The recovery of human EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    97

    92-104


    Platelet-poor plasma (n=4)

    89

    82-95









  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected


    500

    2.380

    2.452

    2.416

    2.306


    250

    1.371

    1.376

    1.374

    1.264


    125

    0.731

    0.722

    0.727

    0.617


    62.5

    0.452

    0.435

    0.444

    0.334


    31.2

    0.273

    0.264

    0.269

    0.159


    15.6

    0.188

    0.195

    0.192

    0.082


    7.8

    0.146

    0.152

    0.149

    0.039


    0

    0.108

    0.111

    0.110










  • 本试剂盒所含材料：


    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human EGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled EGF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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