首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人二肽基肽酶Ⅳ(DPPⅣ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DPP4含量。DPP4是一种广泛存在的跨膜蛋白。其背景上，在糖尿病、肿瘤等疾病中作用受关注。研究机制方面，它能降解多种肽类激素，如GLP-1，影响血糖调节等生理过程。针对DPP4开发抑制剂可提高GLP-1活性，成为糖尿病治疗的重要策略。试剂盒检测范围为1.56 ng/mL-100 ng/mL，灵敏度为0.39 ng/mL。适用于科研领域中对DPPⅣ表达水平的动态监测，例如探究代谢性疾病病理机制、评估药物干预效果或筛选生物标志物等实验场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DPP4; ADCP2; CD26; Dipeptidyl peptidase 4; ADABP; Adenosine deaminase complexing protein 2; ADCP-2; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; TP103; CD antigen CD26
缩写：DPP4
Uniprot No.：P27487
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DPPⅣ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 83-98 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 87-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-105 | |||||
回收率：
| The recovery of human DPPⅣ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 83-106 | ||||
| EDTA plasma (n=4) | 89 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.9312.8522.8922.773 502.4382.4952.4672.348 251.7031.7621.7331.614 12.50.9430.9750.9590.840 6.250.5360.5600.5480.429 3.120.3320.3250.3290.210 1.560.2280.2360.2320.113 00.1180.1200.119 | ||||||
货期：3-5 working days
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