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产品描述：人Dickkopf 1(DKK1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DKK1含量。DKK1是一种重要靶点。它是分泌性糖蛋白，在胚胎发育、组织稳态维持等生理过程中发挥作用。研究发现其异常表达与肿瘤生长、骨代谢紊乱等病理进程相关，机制涉及调控Wnt信号通路等，是疾病治疗和药物研发的潜在靶点。试剂盒检测范围为62.5 pg/mL-4000pg/mL，灵敏度为15.6 pg/mL。支持科研人员高效开展DKK1在细胞信号通路研究、疾病模型验证或生物标志物探索中的应用，特别适用于癌症生物学、骨病机制或免疫相关疾病的分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Dickkopf 1 ELISA Kit; Dickkopf 1 homolog ELISA Kit; Dickkopf 1 like ELISA Kit; Dickkopf homolog 1 ELISA Kit; Dickkopf like protein 1 ELISA Kit; Dickkopf related protein 1 ELISA Kit; Dickkopf WNT signaling pathway inhibitor 1 ELISA Kit; Dickkopf-1 ELISA Kit; Dickkopf-related protein 1 ELISA Kit; DKK 1 ELISA Kit; Dkk-1 ELISA Kit; Dkk1 ELISA Kit; DKK1_HUMAN ELISA Kit; hDkk 1 ELISA Kit; hDkk-1 ELISA Kit; SK ELISA Kit
缩写：DKK1
Uniprot No.：O94907
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DKK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 96-103 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of human DKK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 92 | 92-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.6012.5872.5942.468 20001.8271.7761.8021.676 10001.2491.2281.2391.113 5000.7430.7350.7390.613 2500.4470.4240.4360.310 1250.2740.2660.2700.144 62.50.1930.1880.1910.065 00.1280.1240.126 | ||||||
货期：3-5 working days
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