Rat Cystatin C,Cys-C ELISA Kit

中文名： 大鼠半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)ELISA Kit

货号: IPD-E08385r

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
大鼠半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、匀浆样本中的CST3含量。CST3 即半胱氨酸蛋白酶抑制剂 C，是一种内源性半胱氨酸蛋白酶抑制剂。在人体生理中起稳定蛋白酶平衡等作用。研究发现其与神经系统疾病、肾脏疾病等密切相关，如可作为评估肾小球滤过功能指标，机制可能与调节体内蛋白酶活性和炎症反应等有关。试剂盒检测范围为0.312 ng/ml-20 ng/ml，灵敏度为0.078 ng/ml。适用于科研领域中对肾脏疾病模型、药物代谢研究或组织损伤相关实验中Cys-C表达水平的动态监测，帮助研究者分析其与肾功能状态、炎症进程或细胞凋亡的关联性。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Cst3 ELISA Kit; Cystatin-C ELISA Kit; Cystatin-3 ELISA Kit
缩写：
CST3
Uniprot No.：
P14841
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, homogenates
检测范围：
0.312 ng/ml-20 ng/ml
灵敏度：
0.078 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)2.4902.5502.5882.4672.5272.555SD0.0310.0390.0460.0410.0480.056CV(%)3.4074.2254.9414.5305.2296.061

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:2

Average %

Range %

93-99

1:4

Average %

Range %

90-96

1:8

Average %

Range %

91-104

1:16

Average %

Range %

89-95

回收率：

The recovery of rat Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
20	2.564	2.587	2.576	2.404
10	1.923	1.934	1.929	1.757
5	1.329	1.368	1.349	1.177
2.5	0.912	0.937	0.925	0.753
1.25	0.582	0.598	0.590	0.418
0.625	0.412	0.436	0.424	0.252
0.312	0.311	0.317	0.349	0.177
0	0.170	0.174	0.172