Human Thrombin activatable fibrinolysis inhibitor,TAFI ELISA Kit

中文名： 人纤溶抑制因子/凝血酶激活的纤溶抑制物(TAFI)ELISA Kit

货号: IPD-E08778h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人纤溶抑制因子/凝血酶激活的纤溶抑制物(TAFI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血浆、组织匀浆样本中的CPB2含量。CPB2 即羧肽酶 B2，是一种血浆酶。它可使血浆中缓激肽、补体 C5a 等失活，在凝血、炎症等生理病理过程发挥重要作用。研究多聚焦其在血栓形成、脓毒症等疾病机制，探索以其为靶点开发治疗药物。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，灵敏度为7.81 ng/mL。适用于心血管疾病模型、凝血功能研究或纤维蛋白代谢机制等科研领域，为探究TAFI在血栓调节、纤维蛋白溶解动态平衡中的生物学功能提供可靠工具，支持科研人员开展血浆或组织样本中TAFI的定量分析，满足基础医学研究与药物开发中对凝血 - 纤溶系统动态监测的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CPB2 ELISA Kit; Carboxypeptidase B2 ELISA Kit; EC 3.4.17.20 ELISA Kit; Carboxypeptidase U ELISA Kit; CPU ELISA Kit; Plasma carboxypeptidase B ELISA Kit; pCPB ELISA Kit; Thrombin-activable fibrinolysis inhibitor ELISA Kit; TAFI ELISA Kit
缩写：
CPB2
Uniprot No.：
Q96IY4
种属：
Homo sapiens (Human)
样本类型：
plasma, tissue homogenates
检测范围：
31.25 ng/mL-2000 ng/mL
灵敏度：
7.81 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	254.184	245.488	223.560	229.726	230.786	211.250
SD	0.038	0.033	0.038	0.041	0.044	0.048
CV(%)	13.194	11.186	12.063	13.269	14.286	14.634

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TAFI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:2

Average %

Range %

94-108

1:4

Average %

Range %

92-99

1:8

Average %

Range %

90-99

1:16

Average %

Range %

84-96

回收率：

The recovery of human TAFI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	98	92-104
EDTA plasma (n=4)	92	89-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.





ng/ml	OD1	OD2	Average
2000	0.084	0.085	0.085
1000	0.130	0.131	0.131
500	0.194	0.182	0.188
250	0.299	0.287	0.293
125	0.515	0.492	0.504
62.5	0.911	0.923	0.917
31.25	1.346	1.447	1.397
0	2.425	2.528	2.477