首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人癌胚抗原相关细胞粘附分子1(CEACAM1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CEACAM1含量。CEACAM1即癌胚抗原相关细胞粘附分子 1，是免疫球蛋白超家族成员。它可参与细胞间粘附、信号传导等。在肿瘤中，它影响肿瘤细胞生长、转移及免疫逃逸。目前研究聚焦于其在肿瘤微环境中调控机制，有望为肿瘤治疗提供新靶点与思路。试剂盒检测范围为0.78 ng/mL-50 ng/mL，灵敏度为0.195 ng/mL。适用于体外科研实验中定量分析该分子在肿瘤发生机制、免疫调控通路或药物干预研究中的表达水平变化，尤其适合癌症模型动物血清、患者来源组织匀浆或细胞培养上清等样本的检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Antigen CD66 ELISA Kit; BGP 1 ELISA Kit; BGP ELISA Kit; BGP-1 ELISA Kit; BGPI ELISA Kit; Biliary glycoprotein 1 ELISA Kit; Biliary glycoprotein adhesion molecule ELISA Kit; Biliary glycoprotein ELISA Kit; Carcinoembryonic antigen related cell adhesion molecule 1 ELISA Kit; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 1 ELISA Kit; CD66a ELISA Kit; CD66a antigen ELISA Kit; CEACAM1 ELISA Kit; CEAM1_HUMAN ELISA Kit; meconium antigen 100 ELISA Kit
缩写：CEACAM1
Uniprot No.：P13688
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CEACAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of human CEACAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-103 | ||||
| EDTA plasma (n=4) | 95 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.5802.5422.5612.362 251.7741.8001.7871.588 12.51.0551.1041.0800.881 6.250.6280.6350.6320.433 3.120.5140.5070.5110.312 1.560.3680.3610.3650.166 0.780.2730.2840.2790.080 00.2010.1970.199 | ||||||
货期：3-5 working days
×
