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Human E-Cadherin,E-Cad ELISA Kit

  • 中文名： 人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA Kit
货号: IPD-E04519h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的CDH1含量。CDH1是重要靶点，它所编码的E-钙黏蛋白在维持上皮细胞极性和黏附等方面起关键作用。其功能异常与肿瘤发生发展密切相关，如促进肿瘤细胞侵袭转移。目前围绕其开展的研究，旨在揭示其在肿瘤中的作用机制，为开发针对性抗癌疗法提供依据。试剂盒检测范围为0.312 ng/ml-20 ng/ml，灵敏度为0.078 ng/ml。该产品适用于肿瘤微环境研究、上皮 - 间质转化（EMT）机制探索、组织工程模型构建等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Arc 1 ELISA Kit; CADH1_HUMAN ELISA Kit; Cadherin 1 ELISA Kit; cadherin 1 type 1 E-cadherin ELISA Kit; Cadherin-1 ELISA Kit; Cadherin1 ELISA Kit; CAM 120/80 ELISA Kit; CD 324 ELISA Kit; CD324 ELISA Kit; CD324 antigen ELISA Kit; cdh1 ELISA Kit; CDHE ELISA Kit; E-Cad/CTF3 ELISA Kit; E-cadherin ELISA Kit; ECAD ELISA Kit; Epithelial cadherin ELISA Kit; epithelial calcium dependant adhesion protein ELISA Kit; LCAM ELISA Kit; Liver cell adhesion molecule ELISA Kit; UVO ELISA Kit; Uvomorulin ELISA Kit
  • 缩写：
    CDH1
  • Uniprot No.：
    P12830
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/ml -20 ng/ml
  • 灵敏度：
    0.078 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:20

    Average %

    91


    Range %

    83-99


    1:40

    Average %

    96


    Range %

    93-99


    1:80

    Average %

    102


    Range %

    97-107


    1:160

    Average %

    88


    Range %

    83-93


  • 回收率：

    The recovery of human E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    91

    85-97


    EDTA plasma (n=4)

    98

    95-101


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    2.290

    2.399

    2.345

    2.204


    10

    1.864

    1.746

    1.805

    1.664


    5

    1.384

    1.502

    1.443

    1.302


    2.5

    0.981

    1.056

    1.019

    0.878


    1.25

    0.644

    0.623

    0.634

    0.493


    0.625

    0.389

    0.379

    0.384

    0.243


    0.312

    0.271

    0.269

    0.270

    0.129


    0

    0.136

    0.145

    0.141




  • 货期：
    3-5 working days

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