首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人巨噬细胞来源的趋化因子(MDC/CCL22)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CCL22含量。CCL22是一个靶点。它在免疫调节等生理过程里有重要作用。研究发现，CCL22与多种疾病相关，如肿瘤等，其机制可能是通过与相应受体结合，影响免疫细胞的迁移、活化等，进而参与疾病的发生发展。对它的研究或为相关疾病治疗带来新思路。试剂盒检测范围为15.625 pg/mL-1000 pg/mL，灵敏度为10.324 pg/mL。适用于体外定量检测多种生物样本中的蛋白表达量，能够满足炎症机制研究、药物干预效果评估及生物标志物筛选等科研需求。例如，可通过检测细胞因子诱导后的巨噬细胞培养上清中MDC/CCL22分泌水平，探索免疫调控通路；或分析肿瘤组织匀浆中趋化因子浓度与免疫细胞浸润的相关性，为疾病机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A 152E5.1 ELISA Kit; ABCD 1 ELISA Kit; ABCD1 ELISA Kit; C C motif chemokine 22 ELISA Kit; CC chemokine STCP 1 ELISA Kit; CC chemokine STCP-1 ELISA Kit; ccl 22 ELISA Kit; Ccl22 ELISA Kit; CCL22_HUMAN ELISA Kit; Chemokine (C C motif) ligand 22 ELISA Kit; DC/B CK ELISA Kit; DCBCK ELISA Kit; Macrophage-derived chemokine ELISA Kit; MDC ELISA Kit; MDC(1-69) ELISA Kit; MDC(7-69) ELISA Kit; MGC34554 ELISA Kit; SCYA22 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 22 ELISA Kit; Small inducible cytokine subfamily A; member 22 ELISA Kit; Small-inducible cytokine A22 ELISA Kit; STCP 1 ELISA Kit; STCP1 ELISA Kit; Stimulated T cell chemotactic protein 1 ELISA Kit; Stimulated T-cell chemotactic protein 1 ELISA Kit
缩写：CCL22
Uniprot No.：O00626
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.625 pg/mL-1000 pg/mL
灵敏度：10.324 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MDC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-101 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 94-112 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of human MDC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-93 | ||||
| EDTA plasma (n=4) | 89 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.5342.4212.4782.349 5001.9561.7661.8611.732 2501.1471.0871.1170.988 1250.6020.6110.6070.478 62.50.3410.3310.3360.207 31.250.2450.2620.2540.125 15.6250.1980.2010.2000.071 00.1340.1240.129 | ||||||
货期：3-5 working days
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