Human macrophage inflammatory protein 5,MIP-5 ELISA Kit

中文名： 人巨噬细胞炎性蛋白5(MIP-5)ELISA Kit

货号: IPD-E07997h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人巨噬细胞炎性蛋白5(MIP-5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL15含量。CCL15，又称巨噬细胞抑制蛋白5（MIP-5）、白细胞趋化因子1（Lkn-1）和人CC趋化因子2（HCC-2），是一种小细胞因子，属于CC趋化因子家族。CCL15在肝脏、小肠、结肠和肺的某些白细胞和巨噬细胞中表达，具有趋化中性粒细胞、单核细胞和淋巴细胞的特性，通过与CCR1和CCR3受体结合，在免疫细胞募集和炎症性疾病发展中起关键作用。研究机制表明，CCL15在肿瘤微环境中通过调节相关基因表达，影响肿瘤生长和转移。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.81 pg/mL。该产品为炎症机制研究、疾病模型评估及药物干预实验提供有效工具，特别适用于探索MIP - 5在病理过程中的调控网络、验证体外细胞实验数据或评估动物模型治疗效果。配套提供标准品、预混试剂及操作流程说明，支持常规实验室开展定量检测，满足科研领域对趋化因子信号通路研究的实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
C-C motif chemokine 15 ELISA Kit; C-C motif chemokine ligand 15 ELISA Kit; CC chemokine 3 ELISA Kit; CCL15 ELISA Kit; CCL15(29-92) ELISA Kit; CCL15_HUMAN ELISA Kit; Chemokine (C C motif) ligand 15 ELISA Kit; Chemokine CC-2 ELISA Kit; Chemokine CC2 ELISA Kit; HCC-2 ELISA Kit; HCC2 ELISA Kit; HMRP 2B ELISA Kit; HMRP2B ELISA Kit; Leukotactin 1 ELISA Kit; Leukotactin-1 ELISA Kit; LKN-1 ELISA Kit; LKN1 ELISA Kit; Macrophage inflammatory protein 5 ELISA Kit; MIP 1D ELISA Kit; MIP-1 delta ELISA Kit; MIP-1D ELISA Kit; MIP-5 ELISA Kit; MIP1 delta ELISA Kit; MIP1d ELISA Kit; MIP5 ELISA Kit; MRP 2B ELISA Kit; Mrp-2b ELISA Kit; Mrp2b ELISA Kit; NCC 3 ELISA Kit; NCC-3 ELISA Kit; NCC3 ELISA Kit; New CC chemokine 3 ELISA Kit; OTTHUMP00000163955 ELISA Kit; SCYA15 ELISA Kit; SCYL3 ELISA Kit; Small inducible cytokine A15 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys); member 15 ELISA Kit; Small-inducible cytokine A15 ELISA Kit; SY15 ELISA Kit
缩写：
CCL15
Uniprot No.：
Q16663
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/mL-2000 pg/mL
灵敏度：
7.81 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)244.575250.280257.432237.463249.328256.000SD0.0240.0310.0370.0260.0350.041CV(%)3.3524.2584.9803.7094.8215.541

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:4

Average %

Range %

81-89

1:8

Average %

Range %

91-99

1:16

Average %

Range %

83-92

1:32

Average %

105

Range %

101-109

回收率：

The recovery of human MIP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
Heparin plasma (n=4)	91	89-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.652	2.701	2.677	2.505
1000	1.945	1.932	1.939	1.767
500	1.213	1.204	1.209	1.037
250	0.741	0.735	0.738	0.566
125	0.451	0.432	0.442	0.270
62.5	0.330	0.342	0.336	0.164
31.25	0.266	0.257	0.262	0.090
0	0.170	0.173	0.172