Human angiopoietin-like protein 3,ANGPTL3 ELISA Kit

中文名： 人血管生成素样蛋白3(ANGPTL3)ELISA Kit

货号: IPD-E11724h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人血管生成素样蛋白3(ANGPTL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANGPTL3含量。ANGPTL3是一种颇具研究价值的靶点。它属于血管生成素样蛋白家族，在脂质代谢中发挥关键作用。研究发现，抑制ANGPTL3可调节甘油三酯、胆固醇等脂质水平，有望为治疗高脂血症、心血管疾病等开辟新途径，目前正得到广泛深入的研究。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。适用于基础研究领域，例如探究ANGPTL3在脂代谢异常、动脉粥样硬化等疾病中的分子机制，评估动物模型或细胞实验中的蛋白表达水平变化，以及筛选调控ANGPTL3的潜在药物靶点。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
ANG 5 ELISA Kit; ANG-5 ELISA Kit; ANG5 ELISA Kit; Angiopoietin 5 ELISA Kit; Angiopoietin like 3 ELISA Kit; Angiopoietin related protein 3 ELISA Kit; Angiopoietin-5 ELISA Kit; Angiopoietin-like protein 3 ELISA Kit; Angiopoietin-related protein 3 ELISA Kit; ANGL3_HUMAN ELISA Kit; ANGPT5 ELISA Kit; ANGPTL3 ELISA Kit; ANL3 ELISA Kit; FHBL2 ELISA Kit; OTTHUMP00000010719 ELISA Kit; UNQ153/PRO179 ELISA Kit
缩写：
ANGPTL3
Uniprot No.：
Q9Y5C1
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)639.119656.229645.069632.426624.245636.144SD0.0370.0400.0330.0450.0520.046CV(%)4.7745.0134.2155.8756.8875.966

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ANGPTL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:10	Average %	90
	Range %	80-110
1:20	Average %	97
	Range %	91-105
1:40	Average %	95
	Range %	90-105
1:80	Average %	93
	Range %	86-98

回收率：

The recovery of human ANGPTL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	85-98
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
5000	2.648	2.689	2.669	2.583
2500	2.235	2.206	2.221	2.135
1250	1.442	1.468	1.455	1.369
625	0.806	0.817	0.812	0.726
312.5	0.346	0.369	0.358	0.272
156	0.143	0.151	0.147	0.061
78	0.112	0.116	0.114	0.028
0	0.082	0.089	0.086