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Mouse Albumin (Alb)ELISA Kit

  • 中文名: 小鼠白蛋白ELISA Kit
货号: IPD-E13878m
Price: ¥2500
数量:
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  • 产品描述:小鼠白蛋白酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的ALB含量。ALB即白蛋白,是血浆中含量最丰富的蛋白质。在药物研究中常作为靶点,其背景在于能与多种内源性和外源性物质结合。研究机制主要围绕利用其结合特性,开发与之结合的药物,以实现药物的运输、控释等,提升药物疗效与安全性。试剂盒检测范围为0.781 μg/mL-200 μg/mL,灵敏度为0.110 μg/mL。该产品适用于肝脏疾病模型研究、代谢综合征分析、药物毒性评估及生物标志物筛选等科研场景本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:Alb 1 ELISA Kit; alb ELISA Kit; ALBU_MOUSE ELISA Kit; Albumin 1 ELISA Kit; Albumin ELISA Kit; Serum albumin ELISA Kit

  • 缩写:ALB

  • Uniprot No.:P07724

  • 种属:Mus musculus (Mouse)

  • 样本类型:serum, plasma, tissue homogenates

  • 检测范围:0.781 μg/mL-200 μg/mL

  • 灵敏度:0.110 μg/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Metabolism

  • 测定原理:quantitative

  • 测定方法:Competitive

  • 精密度:

     Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度:

     To assess the linearity of the assay, samples were spiked with high concentrations of mouse albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


  • 标准曲线:

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


  • 本试剂盒所含材料:

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    • A micro ELISA plate --- The 96-well plate has been pre-coated with the anti-mouse Alb antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled Alb (100 x concentrate) (60 μl/bottle)

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (20 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (20 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料:

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      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator that can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期:3-5 working days

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