Human Activin A,ACV-A ELISA Kit

中文名： 人活化素A(ACV-A)ELISA Kit

货号: IPD-E04486h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人活化素A(ACV-A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACV-A含量。人活化素A（ACV-A）是TGF-β超家族成员，参与生殖、胚胎发育、红细胞分化等过程。其研究机制涉及调控细胞增殖、凋亡和炎症反应，与多种疾病如肿瘤、炎症性肠病等发病机制相关。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于基础科研中探究ACV - A在生殖生物学、炎症反应、纤维化疾病及肿瘤微环境中的表达调控机制，尤其适合评估病理模型或药物干预后体液或组织内ACV - A的动态变化，为信号通路研究、生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
ACV-A
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/mL-1000 pg/mL
灵敏度：
3.9 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)125.907124.505129.620124.680126.082125.731SD0.0370.0350.0420.0450.0480.049CV(%)3.5323.3663.9304.3234.5764.681

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

80-92

1:2

Average %

Range %

90-99

1:4

Average %

Range %

92-101

1:8

Average %

105

Range %

100-110

回收率：

The recovery of human ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	93	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	3.254	3.125	3.190	3.072
500	2.329	2.414	2.372	2.254
250	1.588	1.626	1.607	1.489
125	1.098	1.040	1.069	0.951
62.5	0.677	0.634	0.656	0.538
31.2	0.395	0.377	0.386	0.268
15.6	0.206	0.198	0.202	0.084
0	0.117	0.118	0.118