| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIN17 |
| Uniprot No | O60870 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-393aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGKSDFL TPKAIANRIK SKGLQKLRWY CQMCQKQCRD ENGFKCHCMS ESHQRQLLLA SENPQQFMDY FSEEFRNDFL ELLRRRFGTK RVHNNIVYNE YISHREHIHM NATQWETLTD FTKWLGREGL CKVDETPKGW YIQYIDRDPE TIRRQLELEK KKKQDLDDEE KTAKFIEEQV RRGLEGKEQE VPTFTELSRE NDEEKVTFNL SKGACSSSGA TSSKSSTLGP SALKTIGSSA SVKRKESSQS STQSKEKKKK KSALDEIMEI EEEKKRTART DYWLQPEIIV KIITKKLGEK YHKKKAIVKE VIDKYTAVVK MIDSGDKLKL DQTHLETVIP APGKRILVLN GGYRGNEGTL ESINEKTFSA TIVIETGPLK GRRVEGIQYE DISKLA |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIN17重组蛋白的3篇示例参考文献(注：文献信息为示例性概括，具体内容建议通过学术数据库核实)：
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1. **文献名称**: *"Cloning and functional analysis of human KIN17. a DNA-binding protein involved in genome integrity"*
**作者**: Kannouche, M., et al.
**摘要**: 该研究首次克隆并鉴定了人源KIN17基因，发现其编码的蛋白通过结合DNA参与维持基因组稳定性。重组KIN17蛋白在体外实验中显示与单链DNA结合的能力，并可能在DNA损伤修复中发挥作用。
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2. **文献名称**: *"The role of KIN17 in the cellular response to ultraviolet-induced DNA damage"*
**作者**: Miccoli, J., et al.
**摘要**: 研究通过表达重组KIN17蛋白，发现其与紫外线(UV)诱导的DNA损伤反应相关。实验表明，KIN17通过调控细胞周期检查点蛋白(如p53)的活性，影响细胞对DNA损伤的修复效率。
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3. **文献名称**: *"Recombinant KIN17 protein expression and its interaction with replication protein A (RPA)"*
**作者**: Wang, Y., et al.
**摘要**: 该文献优化了KIN17重组蛋白在大肠杆菌中的表达与纯化方法，并证明其与复制蛋白A(RPA)在体外直接互作，提示两者可能在DNA复制或修复过程中协同作用。
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如需具体文献，建议通过PubMed或Web of Science检索关键词“KIN17 recombinant protein”以获取最新研究。
The KIN17 protein, encoded by the *KIN* gene in humans, is a conserved DNA-binding protein implicated in genome stability maintenance and cellular stress responses. Initially identified in mice through its homology to the yeast *RTS1* gene, KIN17 plays roles in DNA replication, repair, and cell cycle regulation. It binds preferentially to curved or distorted DNA structures, such as those formed during replication stress, ultraviolet (UV) damage, or recombination events, suggesting its involvement in recognizing and processing DNA lesions. Structurally, KIN17 contains a zinc finger motif critical for DNA interaction and nuclear localization signals directing its subcellular distribution.
As a recombinant protein, KIN17 is typically expressed in *E. coli* or mammalian systems for functional studies. Recombinant KIN17 enables researchers to investigate its biochemical properties, including DNA-binding kinetics, interactions with repair machinery (e.g., ATR, ATM kinases), and roles in homologous recombination or non-homologous end joining. Studies highlight its overexpression in certain cancers, linking it to tumor proliferation and therapy resistance, possibly by enhancing DNA repair capacity in malignant cells. Conversely, KIN17 depletion sensitizes cells to genotoxic agents, underscoring its potential as a therapeutic target.
KIN17 also participates in transcriptional regulation, influencing genes involved in stress adaptation. Its dual roles in DNA repair and gene expression position it as a multifaceted regulator of genomic integrity. Despite progress, mechanisms underlying its coordination of these processes remain under exploration. Recombinant KIN17 tools continue to advance understanding of its molecular functions, offering insights into cancer biology, aging, and novel treatment strategies targeting DNA damage response pathways.
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