| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | JSRP1 |
| Uniprot No | Q96MG2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-331aa |
| 氨基酸序列 | MSMTTRAWEE LDGGLGSCQA LEDHSALAET QEDRASATPR LADSGSVPHD SQVAEGPSVD TRPKKMEKEP AARGTPGTGK ERLKAGASPR SVPARKKAQT APPLQPPPPP PALSEELPWG DLSLNKCLVL ASLVALLGSA FQLCRDAVPG EAALQARVPE PWVPPSSAPR EPSSPLPKFE AQAPPSAPPA PRAEAEVRPK IPGSREAAEN DEEEPGEATG EAVREDRVTL ADRGPKERPR REGKPRKEKP RKEERPKKER PRKEERPRAA REPREALPQR WESREGGHRP WARDSRDAEP RKKQAWVSPR RPDEEQRPGS RQKLRAGKGR D |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于JSRP1重组蛋白的虚构参考文献示例，基于学术文献的常见结构整理：
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1. **文献名称**：*Cloning and Expression of JSRP1 Recombinant Protein in E. coli*
**作者**：Li, X., & Zhang, Y.
**摘要**：本研究成功克隆了JSRP1基因，并利用大肠杆菌表达系统实现了高效可溶性表达。通过亲和层析纯化获得高纯度蛋白，Western blot验证其特异性。实验表明重组JSRP1在体外能调控钙离子通道活性，为后续功能研究奠定基础。
2. **文献名称**：*Structural Characterization of JSRP1 and Its Role in Cellular Apoptosis*
**作者**：Wang, H., et al.
**摘要**：采用X射线晶体学解析了JSRP1重组蛋白的三维结构，揭示其与Bcl-2家族蛋白的相互作用界面。功能实验显示，JSRP1过表达显著抑制HEK293细胞凋亡，提示其可能通过线粒体途径参与凋亡调控。
3. **文献名称**：*JSRP1 Recombinant Protein Enhances Skeletal Muscle Regeneration in Murine Models*
**作者**：Garcia, R., et al.
**摘要**：在小鼠肌肉损伤模型中，注射JSRP1重组蛋白可加速肌纤维修复，并上调成肌细胞分化标记物MyoD。蛋白质组学分析表明，JSRP1通过激活PI3K/AKT通路促进肌肉再生。
4. **文献名称**：*Optimization of JSRP1 Recombinant Protein Purification for Therapeutic Applications*
**作者**：Chen, L., & Kim, S.
**摘要**：开发了一种新型两步层析法(镍柱联合分子筛)，显著提高JSRP1重组蛋白的回收率和稳定性。体外实验证实纯化后的蛋白具有低内毒素水平，符合生物制剂标准，为药物开发提供工艺参考。
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注：以上内容为模拟生成，实际文献需通过学术数据库检索确认。如需真实文献，建议在PubMed或Web of Science中搜索“JSRP1 recombinant protein”或相关关键词。
JSRP1 (Junction Stabilizing and Regulatory Protein 1) is a recombinant protein engineered to study and modulate cellular junctions, particularly in contexts involving epithelial or endothelial barrier function. Derived from naturally occurring junctional proteins, JSRP1 is designed to target specific components of tight junctions (TJs) or adherens junctions (AJs), which are critical for maintaining tissue integrity, cell polarity, and signal transduction. Its development stems from growing interest in understanding how junctional complexes regulate pathological processes, such as cancer metastasis, inflammatory disorders, and vascular leakage syndromes.
The recombinant JSRP1 typically incorporates functional domains from proteins like claudins, occludin, or cadherins, fused with tags (e.g., His-tag, FLAG) for purification and detection. It is expressed in bacterial or mammalian systems, ensuring proper folding and post-translational modifications. Researchers utilize JSRP1 to investigate mechanisms of junction assembly/disassembly, often through competitive binding assays or as a tool to disrupt endogenous interactions in vitro. In disease models, JSRP1 has shown potential in restoring barrier function in conditions like inflammatory bowel disease (IBD) or sepsis-induced vascular dysfunction by stabilizing intercellular contacts.
Recent studies also explore its role in cancer, where dysregulated cell junctions facilitate tumor invasion. By interfering with oncogenic signaling at junctions, JSRP1 may inhibit metastasis. However, challenges remain in optimizing its bioavailability and specificity for therapeutic applications. Overall, JSRP1 serves as a versatile research tool, bridging structural biology and translational studies aimed at junction-related pathologies.
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