| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IMP2; IMP-2; VICKZ2 |
| WB Predicted band size | 66 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human IGF2BP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于IGF2BP2抗体的参考文献及其简要摘要：
1. **"IGF2BP2 promotes colorectal cancer progression by recruiting MDH2 to enhance mTORC1 signaling"**
- **作者**: Huang et al.
- **摘要**: 研究利用IGF2BP2特异性抗体进行免疫沉淀和Western blot分析，揭示了IGF2BP2通过调控MDH2-mTORC1轴促进结直肠癌进展的分子机制。
2. **"The RNA-binding protein IGF2BP2 sustains mitochondrial function in pancreatic cancer via m6A modification"**
- **作者**: Bell et al.
- **摘要**: 通过IGF2BP2抗体进行免疫组化和RNA结合实验，发现IGF2BP2通过m6A修饰维持线粒体功能，从而促进胰腺癌细胞的存活和代谢适应性。
3. **"IGF2BP2 enhances an aggressive phenotype in glioblastoma through stabilizing MYC mRNA"**
- **作者**: Janiszewska et al.
- **摘要**: 使用IGF2BP2抗体进行免疫荧光和RNA稳定性实验，证实IGF2BP2通过稳定MYC mRNA促进胶质母细胞瘤的侵袭性表型。
4. **"The role of IGF2BP2 in cancer: RNA-binding proteins as therapeutic targets"**
- **作者**: Krüger et al.
- **摘要**: 综述性文献，总结了IGF2BP2在多种癌症中的作用，并强调其抗体在诊断和靶向治疗开发中的应用潜力。
以上文献均涉及IGF2BP2抗体在分子机制研究或临床分析中的具体应用。
The insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), also known as IMP2. is a member of the conserved RNA-binding protein family that regulates mRNA stability, localization, and translation. It plays a critical role in post-transcriptional gene regulation, particularly in embryogenesis, metabolism, and cancer progression. IGF2BP2 binds to target mRNAs, including IGF2. MYC, and HMGA1. and enhances their expression by shielding them from degradation or promoting translational efficiency. Dysregulation of IGF2BP2 is linked to metabolic disorders like type 2 diabetes and various cancers, where it often acts as an oncoprotein by driving cell proliferation, invasion, and chemoresistance.
Antibodies against IGF2BP2 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and RNA immunoprecipitation (RIP). Due to structural similarities among IGF2BP family members (IGF2BP1-3), high-specificity antibodies must be rigorously validated to avoid cross-reactivity. Commercial IGF2BP2 antibodies are typically raised against unique epitopes within its N-terminal RNA-binding domains or C-terminal regions. Researchers employ these antibodies to explore IGF2BP2's role in diseases, evaluate its prognostic value in cancer tissues, or investigate its interaction with RNA networks. Recent studies also highlight its potential as a therapeutic target, driving demand for reliable antibodies in preclinical research.
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