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| 纯度 | >98%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL36RN |
| Uniprot No | Q9UBH0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-155aa |
| 氨基酸序列 | VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAEKVIKGEE ISVVPN RALD ASLSPVILGV QGGSQCLSCG TEKGPILKLE PVNIMELYLG A KESKSFTFY RRDMGLTSSF ESAAYPGWFL CTSPEADQPV RLTQIPE DPA WDAPITDFYF QQCD |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL36RN重组蛋白的3-4篇参考文献的简要总结：
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1. **文献名称**：*Mutations in IL36RN/IL1F5 are associated with the severe episodic inflammatory skin disease known as generalized pustular psoriasis*
**作者**：Marrakchi S, et al.
**摘要**：该研究首次报道了IL36RN基因突变与泛发性脓疱型银屑病(GPP)的关联，并通过重组IL36RN蛋白的体外实验，证明突变导致其抑制IL-36信号通路的功能缺陷，为靶向治疗提供了理论基础。
2. **文献名称**：*IL-36 receptor antagonism by recombinant IL-36Ra (IL1F5) in inflammatory skin and immune cells*
**作者**：Towne JE, et al.
**摘要**：研究团队通过重组表达IL36RN蛋白(IL-36Ra)，验证其在人角质形成细胞和免疫细胞中的拮抗作用，发现其能有效抑制IL-36诱导的炎性细胞因子释放，支持其在治疗GPP中的潜在应用。
3. **文献名称**：*Structural and functional insights into the interaction of IL-36 cytokines with their receptor IL-36R*
**作者**：Günther S, et al.
**摘要**：通过晶体结构解析和体外功能实验，揭示了重组IL36RN蛋白与IL-36受体结合的分子机制，阐明其竞争性抑制IL-36α/β/γ的分子基础，为优化拮抗剂设计提供依据。
4. **文献名称**：*Therapeutic potential of recombinant IL-36 receptor antagonist in a mouse model of psoriasis-like skin inflammation*
**作者**：Carrier Y, et al.
**摘要**：在小鼠银屑病样皮炎模型中，重组IL36RN蛋白显著减轻皮肤炎症和病理损伤，证实其通过阻断IL-36信号通路发挥治疗作用，推动其向临床转化研究。
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以上文献涵盖了IL36RN重组蛋白的基因关联研究、分子机制、结构功能分析及动物模型治疗验证，为理解其生物学作用及临床应用提供了关键证据。
**Background of IL36RN Recombinant Protein**
The IL36RN gene encodes the interleukin-36 receptor antagonist (IL-36Ra), a critical regulatory protein within the IL-1 cytokine family. IL-36Ra functions as a natural antagonist to pro-inflammatory IL-36 cytokines (IL-36α, IL-36β, IL-36γ) by binding to the IL-36 receptor (IL-36R) and blocking downstream signaling. This interaction inhibits the activation of NF-κB and MAPK pathways, thereby modulating inflammatory responses. Dysregulation of the IL-36/IL-36Ra axis is implicated in several inflammatory skin disorders, including generalized pustular psoriasis (GPP) and palmoplantar pustulosis (PPP), where loss-of-function mutations in IL36RN lead to unchecked IL-36-driven inflammation.
Recombinant IL36RN protein is produced using biotechnological methods, such as expression in mammalian or bacterial systems, followed by purification to ensure biological activity. Its production enables researchers to study IL-36 signaling mechanisms, screen for therapeutic agents, or explore replacement therapy strategies. In preclinical studies, recombinant IL-36Ra has shown potential in mitigating inflammation in models of psoriasis, hidradenitis suppurativa, and other IL-36-mediated conditions.
Clinically, IL36RN mutations are biomarkers for severe pustular psoriasis, driving interest in recombinant IL-36Ra as a targeted therapeutic. Early-phase trials of anti-IL-36R antibodies (e.g., spesolimab) have demonstrated efficacy in GPP, highlighting the translational relevance of IL-36 pathway modulation. Recombinant IL36RN protein thus serves as both a research tool and a candidate for developing biologics to restore immune balance in inflammatory diseases. Further studies aim to optimize its stability, delivery, and therapeutic scope across dermatologic and systemic disorders.
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