| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Creb5 |
| Uniprot No | Q8K1L0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-357aa |
| 氨基酸序列 | MSMRPVPGSLSSLLHLHNRQRQPMPASMPGTLPNPTMPGSSAVLMPMERQMSVNSSIMGMQGPNLSNPCASPQVQPMHSEAKMRLKAALTHHPAAMSNGNMSTIGHMMEMMGSRQDQTPHHHLHSHPHQHQTLPPHHPYPHQHQHPAHHPHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHTGNQAQVSPATQQMQPTQTIQPPQPTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVWVMSLEKKAEELTQTNMQLQNEVSMLKNEVAQLKQLLLTHKDCPITAMQKESQGYLSPESSPPASPVPACSQQQVIQHNTITTSSSVSEVVGSSTLSQLTTHRTDLNPIL |
| 预测分子量 | 44.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的3-4篇关于Creb5重组蛋白的参考文献示例(非真实文献，供参考格式及研究方向)：
1. **文献名称**: "Expression and Functional Analysis of Recombinant Creb5 in Mammalian Cells"
**作者**: Zhang L, et al.
**摘要**: 本研究在大肠杆菌系统中成功表达并纯化了人源Creb5重组蛋白，验证了其与DNA中cAMP响应元件的特异性结合能力，并证明其在细胞氧化应激反应中的调控作用。
2. **文献名称**: "Creb5 Recombinant Protein Attenuates Hepatic Fibrosis via TGF-β/Smad Pathway"
**作者**: Wang Y, et al.
**摘要**: 通过构建His标签的Creb5重组蛋白，发现其可抑制肝星状细胞活化，降低TGF-β1介导的胶原沉积，为肝纤维化治疗提供潜在靶点。
3. **文献名称**: "Structural Characterization of Creb5 DNA-Binding Domain by X-ray Crystallography"
**作者**: Tanaka K, et al.
**摘要**: 首次解析了Creb5重组蛋白的DNA结合域晶体结构(2.1Å分辨率)，揭示了其碱性亮氨酸拉链(bZIP)结构域与靶DNA的相互作用机制。
4. **文献名称**: "High-Yield Production of Recombinant Creb5 in Insect Cells and Its Role in Apoptosis Regulation"
**作者**: Chen X, et al.
**摘要**: 利用杆状病毒-昆虫细胞系统实现Creb5重组蛋白的高效表达，功能实验表明其通过调控Bcl-2家族蛋白抑制肿瘤细胞凋亡。
---
**注意**：以上文献为模拟内容，实际研究需通过PubMed/Google Scholar等平台以关键词"Creb5 recombinant protein"检索真实文献。近期研究多聚焦于Creb5在代谢疾病、癌症及发育生物学中的功能机制。
**Background of CREB5 Recombinant Protein**
CREB5 (cAMP Responsive Element-Binding Protein 5) is a member of the CREB/ATF transcription factor family, which regulates gene expression by binding to cAMP-responsive elements (CREs) in DNA. It shares structural homology with other CREB proteins, including a conserved basic leucine zipper (bZIP) domain that facilitates dimerization and DNA binding. CREB5 is broadly expressed in tissues such as the liver, kidney, and brain, and is implicated in cellular processes like proliferation, differentiation, and apoptosis.
Unlike its well-studied relative CREB1. CREB5 exhibits distinct regulatory roles. Studies suggest it may act as both a transcriptional activator and repressor, depending on cellular context and post-translational modifications (e.g., phosphorylation). Its involvement in pathways like MAPK/ERK and PI3K/AKT highlights its role in stress response, metabolism, and oncogenesis. For instance, CREB5 overexpression has been linked to tumor progression in cancers like hepatocellular carcinoma and colorectal cancer.
Recombinant CREB5 proteins are engineered for functional studies, typically produced via bacterial or mammalian expression systems. These proteins retain key domains (e.g., bZIP) and are purified for *in vitro* assays, such as DNA-binding studies, protein interaction analyses, or inhibitor screening. Tagged versions (e.g., His-tag, GST-tag) enable efficient purification and detection.
Research on CREB5 recombinant proteins aids in deciphering its role in diseases and therapeutic targeting. Challenges include understanding isoform-specific functions and post-translational regulation. Overall, CREB5 represents a promising yet under-explored target in transcriptional regulation and disease mechanisms.
×
