| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHS5 |
| Uniprot No | Q8TGV2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1885aa |
| 氨基酸序列 | MATRGNVPAHMQASLPALPAHLQSDTHITAHLASRFHVSLPTARLSSQGLICLNTFTSSTRGPNGDKEGSAMGEPEDLARRAWARLGNRAEDQAFGFFGESGSGKTTVRSHLLSSFLSFSSTPLSSKLSLAAFVFDTLTTTKTTTTQTASKAGLFYELQYDASSNNPTLIGGKLLDHRFERSRISHVPTGERSFHVLYYLLAGTSAAEKSHLGLDGHVNITTAGTGLSRSASVSHKRWRYLGHPTQMKVGINDAEGFQHFKNALRKLEFPRTEIAEICQVLAAILHIGQLEFGTGQATLTAAEESGGYSHEGGETVTVVKNRDTLAIVAAFLGLGVQDLEESLRYKTRTIHRERVTVMLDTKGARENADELATTLYSLLVTYIIESINQRVCAAEDSVANTISIVDFPGFADHSSTGSVLDQLLNNAANESLYNTCLHSIFEKTAEMLESEEVSVPATSYFDNSDAVRGLLKHGNGLLAILDDQTRRGRTDVQFLESLRKRFENKNKAITVGSATSTMPGSNFATTNLAASFTVRHYAGEVDYPVHSLVEENGDVVSGDLMNMIKATKSDFVANLFGQEALNTVSHPAEKTAIVQAQVSSKPLRMPSVSRKKHDQLRRMASRRADRSPAPQEEEPLPGTEEAKVRRTKPTATGLTQGAAAQFLSALDNITKSLTAPNVNNYFVFCLKPNDRRIANQFDSKCVRQQVQMFGIAEISQRLRTADFTIFLPFGEFLGLTNADGGVVGSDREKAQLVLDSKHWPPNEARIGNTGVFLSERCWASIALTGSQAAAYFGGDIGSPSRPDTPGHNPFSDSKARLVGSADGTPGSFYGDEAKGGGYFGSRELDAKSDAGASAFHSGDMFRNLETKEELAEKGNKKKVEEVDVVPVSSSRKRWLAIVYFLTWYLPDFAIKWIGGMKRKDVRTAWREKFAINLLIWLSCGLVVFFIIVFPELICPKQNVYSAAELSAHDGKGKHSAYVAIRGQVFDLGAFMPNHYPKIIPQSSLKKYAGVDATGLFPVQVSALCQGKDGRVDPTVQLDYTATNISGTAAVISSTDANRKYHDFRYFTNDSRPDWFYEQMIMLKANYRKGSIGYTPQYVKTLAKKSKSIAILNDRVYDFTTYNEGGRSVRAPPGEEVPSGVDTDFMDSLVVDLFTQRAGHDVTKYWNALPLDPGLRSRMQLCLDNLFFVGVTDTRNSPRCLFARYILLAVSILLCSVIGFKFFAALQFGGKNVPENLDKFVICQVPAYTEDEDSLRRAIDSAARMRYDDKRKLLIVVCDGMIIGQGNDRPTPRIVLDILGVSETVDPEPLSFESLGEGMKQHNMGKVYSGLYEVQGHIVPFMVVVKVGKPSEVSRPGNRGKRDSQMVIMRFLHRVHYNLPMSPLELEMHHQIRNIIGVNPTFYEFMLQIDADTVVAPDSATRMVSAFLRDTRLIGVCGETSLSNAKSSFITMMQVYEYYISHNLTKAFESLFGSVTCVPGCFTMYRIRAAETGKPLFVSKEIIQDYSEIRVDTLHMKNLLHLGEDRYLTTLLLKYHSKYKTKYIFHAHAWTIAPDSWKVFMSQRRRWINSTVHNLIELIPLQQLCGFCCFSMRFVVFLDLLSTVVAPVTVAYIAYLIVLLATESDVVPLTAFILLGAIYGLQAIIFILRRKWEMIGWMIVYILAMPVFSLGLPLYAFWHMDDFSWGNTRLVRGEHGKQILLSDEGKFGPDSIPKKKWEEYQAELWDAQTQRDDARSELSGYSYGTKSYLPTGSVYGGGYNDTQHLMMAPSRSASQLDMHPTPMYGGGGGHNQSRMSLAPSEMLGSQSNLMMPSGRSVADMEMSDLTGLPTDDMLLNEIRDILRTADLMTVTKKGIKQELERRFNVNLDMKRAYIGSATEAILSGQL |
| 预测分子量 | 209kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNAG重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: *Heterologous Expression and Functional Analysis of CNAG_02598 in Cryptococcus neoformans*
**作者**: Zhang L, et al.
**摘要**: 本研究成功克隆并表达了隐球菌CNAG_02598基因的重组蛋白，通过大肠杆菌表达系统纯化后，发现该蛋白具有金属蛋白酶活性，并参与真菌细胞壁的完整性调控，为抗真菌药物靶点研究提供依据。
2. **文献名称**: *Biochemical Characterization of Recombinant CNAG_06743 as a Virulence Factor*
**作者**: Wang Y, et al.
**摘要**: 通过真核表达系统获得CNAG_06743重组蛋白，证实其在隐球菌侵染宿主过程中通过干扰宿主免疫信号通路(如TLR4/NF-κB)增强病原性，为开发靶向抑制剂奠定基础。
3. **文献名称**: *Immunogenicity Evaluation of CNAG_04560 Recombinant Protein in a Murine Model*
**作者**: Chen H, et al.
**摘要**: 利用昆虫细胞表达系统制备CNAG_04560重组蛋白，动物实验表明其能诱导强烈的Th1型免疫反应，显著提高小鼠对隐球菌感染的存活率，提示其作为疫苗候选分子的潜力。
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**说明**：以上文献为示例，实际研究中请根据具体CNAG编号和研究方向检索真实文献(如PubMed或NCBI数据库)。若需进一步协助文献检索，请提供更具体的研究背景或基因编号。
**Background of CNAG Recombinant Protein**
CNAG (Candida albicans N-Acetylglucosamine) recombinant protein is a genetically engineered protein derived from the opportunistic fungal pathogen *Candida albicans*. This protein is associated with metabolic pathways involved in cell wall biosynthesis, particularly the utilization of N-acetylglucosamine (GlcNAc), a sugar critical for fungal cell membrane integrity and virulence. *C. albicans* is a commensal organism in humans but can cause severe infections in immunocompromised individuals. Understanding its survival mechanisms, including nutrient assimilation and stress adaptation, is key to developing antifungal therapies.
The recombinant CNAG protein is typically produced using heterologous expression systems, such as *Escherichia coli* or yeast, to enable high-yield, purified protein for functional studies. Its structure often includes conserved domains linked to GlcNAc catabolic enzymes, such as kinases or transporters, which facilitate fungal adaptation to host environments. Research on CNAG focuses on elucidating its role in fungal pathogenesis, including biofilm formation, hyphal morphogenesis, and immune evasion.
Studies leveraging CNAG recombinant protein have contributed to identifying antifungal drug targets and designing inhibitors that disrupt GlcNAc metabolism. Additionally, it serves as a tool for probing host-pathogen interactions, such as how immune cells recognize fungal components. Recent advancements in structural biology and proteomics have further clarified its molecular interactions and regulatory mechanisms.
Overall, CNAG recombinant protein represents a vital resource in both basic mycology research and translational applications, bridging gaps between understanding fungal biology and developing novel therapeutic strategies against candidiasis and other fungal infections.
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