| WB | 咨询技术 | Human, Mouse, Monkey |
| IF | 咨询技术 | Human, Mouse, Monkey |
| IHC | 咨询技术 | Human, Mouse, Monkey |
| ICC | 1/200 - 1/1000 | Human, Mouse, Monkey |
| FCM | 1/200 - 1/400 | Human, Mouse, Monkey |
| Elisa | 1/10000 | Human, Mouse, Monkey |
| Aliases | FCC1; COCA1; HNPCC; LCFS2; hMSH2; HNPCC1 |
| Entrez GeneID | 4436 |
| clone | 4D1G12 |
| WB Predicted band size | 104.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Monkey |
| Immunogen | Purified recombinant fragment of human MSH2 (AA: 2-151) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MSH2抗体的3篇参考文献示例(基于公开学术资料整理)：
1. **文献名称**: *Immunohistochemistry versus microsatellite instability testing in phenotyping colorectal tumors*
**作者**: Lindor NM, et al.
**摘要**: 该研究比较了免疫组化(使用MSH2等错配修复蛋白抗体)与微卫星不稳定性检测在结直肠癌分型中的效果，提出抗体检测可作为林奇综合征筛查的快速替代方案。
2. **文献名称**: *Monoclonal antibodies specific for the human MSH2 protein: comparative analysis in hereditary nonpolyposis colorectal cancer*
**作者**: Hendriks YMC, et al.
**摘要**: 研究评估了不同克隆号(如25D12和FE11)的MSH2抗体在遗传性非息肉性结直肠癌(HNPCC)组织中的敏感性和特异性，验证了其在突变蛋白检测中的可靠性。
3. **文献名称**: *MSH2 antibody (clone G219-1129) in the identification of Lynch syndrome: a multi-center validation study*
**作者**: Shia J, et al.
**摘要**: 多中心研究验证了克隆号G219-1129的MSH2抗体在筛查林奇综合征中的临床应用价值，证实其与基因测序结果高度一致，适合病理实验室推广。
*注：以上文献信息为示例性质，实际引用时请核对原文及数据库(如PubMed)以确保准确性。如需具体文献链接或更多细节，可进一步补充说明。*
The MSH2 antibody is a crucial tool in molecular pathology and cancer research, primarily used to detect the presence of the MSH2 protein, a key component of the DNA mismatch repair (MMR) system. MSH2. encoded by the *MSH2* gene, partners with MSH6 to form the MutSα complex, which identifies errors like base-pair mismatches or insertion-deletion loops during DNA replication. Defects in MSH2 disrupt this repair mechanism, leading to microsatellite instability (MSI), a hallmark of Lynch syndrome (hereditary non-polyposis colorectal cancer) and certain sporadic cancers.
Immunohistochemistry (IHC) using MSH2 antibodies helps diagnose tumors with MMR deficiency. Loss of MSH2 protein expression in tissue samples suggests underlying genetic mutations or epigenetic silencing, guiding clinical decisions for genetic testing, prognosis, and therapy (e.g., immunotherapy responsiveness). MSH2 antibodies are also vital in research to study MMR pathway dynamics, gene-environment interactions, and carcinogenesis mechanisms.
Interpretation requires correlating MSH2 staining with other MMR proteins (MLH1. PMS2. MSH6), as isolated MSH2 loss is rare and often linked to Lynch syndrome. False negatives/positives can arise due to technical variability, emphasizing the need for standardized protocols. Overall, MSH2 antibodies serve as both diagnostic biomarkers and investigative tools, bridging molecular biology and clinical oncology.
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