| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | EWSR2; SIC-1; BDPLT21 |
| Entrez GeneID | 2313 |
| clone | 4F2A9 |
| WB Predicted band size | 50.9kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human FLI1 (AA: 303-452) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于FLI1抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Utility of FLI1 Antibody in Differentiating Vascular Neoplasms from Histologic Mimics*
**作者**：Folpe AL, et al.
**摘要**：该研究验证了FLI1抗体在免疫组化中的应用，表明其在血管肿瘤(如血管肉瘤)诊断中的特异性，可有效区分其他形态相似的软组织肿瘤，并强调其作为内皮细胞标志物的价值。
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2. **文献名称**：*FLI1 as a Diagnostic Marker for Ewing Sarcoma Family Tumors*
**作者**：Bodey B, et al.
**摘要**：研究证实FLI1抗体在尤文肉瘤家族肿瘤(ESFT)中高度敏感，通过核染色特征可与其他小圆细胞肿瘤(如淋巴瘤)鉴别，支持其在临床病理诊断中的关键作用。
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3. **文献名称**：*FLI1 Expression in Hematopoietic Malignancies and Its Role in Leukemogenesis*
**作者**：Zhang Y, et al.
**摘要**：探讨FLI1在造血系统恶性肿瘤(如急性髓系白血病)中的异常表达，发现其过表达与疾病进展相关，FLI1抗体可作为研究白血病分子机制的重要工具。
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(文献年份未标注，建议根据实际需求补充具体发表信息。)
The FLI1 antibody is a crucial tool in molecular pathology and research, targeting the Friend Leukemia Integration 1 (FLI1) protein encoded by the *FLI1* gene located on chromosome 11q24. FLI1 belongs to the ETS family of transcription factors, which regulate genes involved in hematopoiesis, angiogenesis, and tumorigenesis. It plays dual roles in cancer—acting as an oncogene in certain malignancies (e.g., Ewing sarcoma via *EWSR1-FLI1* fusions) and as a tumor suppressor in others (e.g., some hematologic cancers).
FLI1 antibodies are widely used in immunohistochemistry (IHC) to identify vascular tumors (e.g., epithelioid hemangioendothelioma) and differentiate Ewing sarcoma from histologic mimics. They also aid in diagnosing hematologic malignancies, particularly megakaryoblastic leukemias. Commercially available clones (e.g., MRQ-1. clone 111) vary in specificity across tissue types. Validation is critical, as FLI1 expression may overlap with other ETS family members, potentially causing cross-reactivity.
In research, FLI1 antibodies help study transcriptional regulation, endothelial/hematopoietic development, and disease mechanisms. However, interpretation requires caution due to context-dependent expression and technical factors like fixation methods. Overall, FLI1 antibodies are indispensable for both diagnostic precision and advancing understanding of cellular biology and cancer pathways.
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