| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Cyp3a25 |
| Uniprot No | O09158 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-503aa |
| 氨基酸序列 | MELIPNLSIETWVLLVTSLVLFYIYGTYSHGLFKKLGIPGPKPLPLLGTIFNYYDGMWKFDEDCYKKYGKIWGFYEGPQPILAIMDPEIIKIVLVKECYSVFTNRRFFGPVGFMKKAITISEDEEWKRLRTLLSPTFTSGKLKEMFPIMRQYGDILVRNLRREEEKGEPISMKDIFGAYSMDVITGTSFGVNVDSLNNPQDPFVQKAKKILKFKIFDPFLLSIILFPFLTPIYEMLNFSIFPRDSMNFFKKFVKRMKKERLASNQKNRVDFLQLMMNTQNSKGQESQKALSDLEMAAQAVIFIFGGYDATSTSISLIMYELATHPDVQKKLQDEIDRTLPNKAPVTYDALMDMEYLDMVVNESLRLYPIAIRLERVSKKDVEINGVFIPKGTVVMIPIYPLHRNPEYWPEPQEFCPERFSKENKGNIDPYIYMPFGNGPRNCIGMRFALISIKLAVIGVLQNFTVQPCEETQIPLKISREPIFQPEKPIILKVVSRDKPRTGS |
| 预测分子量 | 65.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYP3A25重组蛋白的3-4条参考文献示例(文献为模拟示例，仅供参考)：
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1. **文献名称**：*"Heterologous Expression and Functional Characterization of Murine CYP3A25 in Escherichia coli"*
**作者**：H. Wang et al.
**摘要**：研究报道了在小鼠CYP3A25重组蛋白的大肠杆菌表达系统中成功实现可溶性表达，并通过亲和层析纯化。功能分析表明，该蛋白可代谢睾酮和硝苯地平，活性依赖NADPH-细胞色素P450还原酶，提示其在药物代谢中的潜在作用。
2. **文献名称**：*"Enzymatic Properties of CYP3A25 Expressed in Baculovirus-Transfected Insect Cells"*
**作者**：T. K. Lee et al.
**摘要**：利用杆状病毒-昆虫细胞系统表达CYP3A25重组蛋白，发现其对咪达唑仑的羟基化活性显著高于其他CYP3A亚型(如CYP3A11)。研究强调了CYP3A25在小鼠肝外组织(如肠道)药物代谢中的特殊性。
3. **文献名称**：*"Comparative Analysis of CYP3A25 and CYP3A4 Substrate Specificity Using Chimeric Proteins"*
**作者**：M. Rodriguez et al.
**摘要**：通过构建CYP3A25与人类CYP3A4的嵌合体蛋白，揭示了二者底物结合口袋的关键差异，解释了CYP3A25对某些类固醇代谢的高效性，为跨物种药物代谢研究提供依据。
4. **文献名称**：*"Development of a CYP3A25-Specific Polyclonal Antibody for Tissue Distribution Studies"*
**作者**：J. Suzuki et al.
**摘要**：研究利用纯化的重组CYP3A25蛋白制备特异性抗体，免疫组化分析显示其在小鼠肾脏和肝脏中高表达，提示CYP3A25可能在全身性药物清除中发挥作用。
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**注意**：以上文献为模拟生成，实际研究中请通过PubMed、SciFinder等学术平台检索真实发表的论文。
**Background of Cyp3a25 Recombinant Protein**
Cytochrome P450 3A25 (Cyp3a25) is a member of the cytochrome P450 superfamily, a group of heme-containing enzymes critical for metabolizing endogenous compounds and xenobiotics, including drugs, toxins, and carcinogens. As part of the CYP3A subfamily, Cyp3a25 is primarily expressed in the liver and intestines of rodents, playing a key role in phase I drug metabolism through oxidation reactions. Its activity influences the pharmacokinetics and bioavailability of numerous therapeutics, impacting drug efficacy and toxicity.
The recombinant Cyp3a25 protein is engineered using heterologous expression systems, such as *E. coli* or insect cell lines (e.g., baculovirus systems), to enable large-scale production for *in vitro* studies. This recombinant form retains enzymatic activity comparable to the native protein, allowing researchers to study substrate specificity, metabolic pathways, and interactions with inhibitors or inducers without interference from other CYP isoforms.
Research on Cyp3a25 is particularly relevant in preclinical drug development, as rodent models are widely used to predict human metabolic outcomes. However, species-specific differences between rodent CYP3A isoforms (e.g., Cyp3a25) and human CYP3A4/5 necessitate careful interpretation of data. Studies on recombinant Cyp3a25 help clarify these interspecies variations, aiding in the extrapolation of animal data to humans. Additionally, this protein is utilized to investigate drug-drug interactions and metabolic stability, supporting the optimization of therapeutic candidates.
Overall, Cyp3a25 recombinant protein serves as a vital tool for advancing mechanistic understanding of drug metabolism while bridging gaps in translational research.
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