| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CREB2; TXREB; CREB-2; TAXREB67 |
| Entrez GeneID | 468 |
| clone | 2A6F12 |
| WB Predicted band size | 38.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ATF4 (AA: 212-351) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ATF4抗体的3篇文献摘要信息，供参考：
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1. **文献名称**：*ATF4 regulates MYC-mediated neuroblastoma cell death upon glutamine deprivation*
**作者**：Xiao, H. et al.
**摘要**：研究利用ATF4抗体通过Western blot和免疫荧光验证了ATF4在神经母细胞瘤细胞中的表达变化，发现ATF4在谷氨酰胺缺乏时通过调控MYC信号通路诱导肿瘤细胞凋亡。
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2. **文献名称**：*The integrated stress response in ischemic kidney injury: ATF4-dependent signaling*
**作者**：Chen, Y. et al.
**摘要**：文章通过ATF4抗体检测缺血再灌注损伤模型中肾脏细胞的ATF4蛋白水平，揭示ATF4通过整合应激反应(ISR)调控氧化应激和细胞存活的分子机制。
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3. **文献名称**：*ATF4 promotes bone sarcoma development via enhancing mitochondrial biogenesis*
**作者**：Wang, L. et al.
**摘要**：研究使用ATF4抗体进行免疫组化和ChIP实验，证明ATF4通过激活线粒体生物合成相关基因促进骨肉瘤的进展，为靶向ATF4的抗癌治疗提供依据。
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4. **文献名称**：*Selective regulation of heme biosynthesis by ATF4 in response to amino acid starvation*
**作者**：Ye, J. et al.
**摘要**：通过ATF4抗体检测氨基酸饥饿条件下的细胞，发现ATF4特异性调控血红素合成通路，揭示了其在代谢适应性中的关键作用。
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以上文献均通过ATF4抗体在特定疾病模型或应激条件下研究其功能，涵盖肿瘤、代谢和器官损伤等领域。如需具体期刊或年份信息可进一步补充。
The ATF4 (Activating Transcription Factor 4) antibody is a crucial tool for studying the role of ATF4. a basic leucine zipper (bZIP) transcription factor involved in cellular stress responses. ATF4 regulates gene expression under conditions such as endoplasmic reticulum (ER) stress, amino acid deprivation, hypoxia, and oxidative stress. It is a key mediator of the integrated stress response (ISR), coordinating adaptive pathways to restore homeostasis or trigger apoptosis if stress persists. ATF4 activates genes involved in metabolism, redox balance, and autophagy, and its dysregulation is linked to cancer, neurodegenerative disorders, and metabolic diseases.
ATF4 antibodies are designed to detect endogenous ATF4 protein in various applications, including Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies are typically raised in hosts like rabbits or mice using immunogens derived from specific ATF4 epitopes. Validation includes testing for specificity via knockout cell lines or siRNA knockdown. Researchers rely on ATF4 antibodies to explore its interaction with partners like C/EBP proteins, its role in stress signaling pathways (e.g., PERK-eIF2α-ATF4 axis), and its therapeutic relevance in disease models. Proper antibody selection requires attention to cross-reactivity, species compatibility, and experimental conditions, as ATF4 expression is often low under basal states but rapidly induced during stress.
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