| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GIG4 |
| Entrez GeneID | 5872 |
| clone | 8E8E2 |
| WB Predicted band size | 22.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Rab13 (AA: 66-200) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇关于Rab13抗体的参考文献及其简要摘要：
1. **"Rab13 regulates membrane trafficking between TGN and recycling endosomes in polarized epithelial cells"**
- **作者**: Marzesco, A.M. et al. (2002)
- **摘要**: 该研究利用特异性Rab13抗体，通过免疫荧光和Western blot分析，揭示了Rab13在上皮细胞极性形成中调控跨高尔基体网络(TGN)与循环内体之间的膜运输机制。
2. **"Rab13 promotes breast cancer progression by modulating EGFR and integrin signalling"**
- **作者**: Ioannou, M.S. et al. (2015)
- **摘要**: 通过Rab13抗体进行免疫组化分析，发现Rab13在侵袭性乳腺癌中高表达，并通过调控EGFR和整合素信号通路促进肿瘤细胞迁移和转移。
3. **"Rab13 directs vesicle trafficking for cell polarity maintenance via the Par3–aPKC complex"**
- **作者**: Zhu, H. et al. (2017)
- **摘要**: 研究利用Rab13抗体进行共免疫沉淀和细胞定位实验，阐明Rab13通过Par3-aPKC复合物调控囊泡运输，维持细胞极性和迁移功能。
4. **"Rab13 knockout in mice alters intestinal barrier function through disrupted tight junction dynamics"**
- **作者**: Wang, Y. et al. (2020)
- **摘要**: 通过Rab13抗体在基因敲除小鼠模型中的免疫印迹和免疫染色分析，证实Rab13缺失导致紧密连接蛋白异常分布，影响肠道屏障完整性。
以上文献展示了Rab13抗体在细胞运输、癌症机制及组织屏障研究中的关键应用。
Rab13. a member of the Rab GTPase family, regulates intracellular vesicle trafficking and membrane dynamics, particularly in processes like cell-cell junction assembly, cell polarity, and protein transport. It plays critical roles in epithelial and endothelial cell functions, influencing cancer metastasis, cell migration, and tight junction dynamics. Dysregulation of Rab13 is linked to pathological conditions, including cancer progression and neurological disorders.
Rab13 antibodies are essential tools for detecting and studying the expression, localization, and function of Rab13 in biological systems. These antibodies are commonly used in techniques such as Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC). They are often generated against specific epitopes, such as the N-terminal or C-terminal regions of Rab13. and may target active (GTP-bound) or inactive (GDP-bound) conformations. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect multiple isoforms or post-translational modifications.
Validating Rab13 antibodies is crucial due to high sequence homology among Rab GTPases. Specificity is typically confirmed using knockout/knockdown controls or recombinant protein assays. Applications span cell biology, cancer research (e.g., studying tumor invasiveness), and neurobiology (e.g., synaptic vesicle trafficking). Commercially available antibodies often include validation data from peer-reviewed studies, aiding researchers in selecting reliable reagents for mechanistic or diagnostic investigations.
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