首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL-23 |
| Uniprot No | Q9NPF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-328aa |
| 氨基酸序列 | IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCSGSTS GSGKPGSGEG STKGRAVPGG SSPAWTQCQQ LSQKLCTLAW SAHPLVGHMD LREEGDEETT NDVPHIQCGD GCDPQGLRDN SQFCLQRIHQ GLIFYEKLLG SDIFTGEPSL LPDSPVGQLH ASLLGLSQLL QPEGHHWETQ QIPSLSPSQP WQRLLLRFKI LRSLQAFVAV AARVFAHGAA TLSP |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL-23重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*IL-23 is critical for induction of organ-specific autoimmune diseases*
**作者**：Cua, D.J., et al.
**摘要**：该研究通过构建IL-23重组蛋白和IL-12缺陷小鼠模型，发现IL-23(而非IL-12)在实验性自身免疫性脑脊髓炎(EAE)中起关键作用，揭示了IL-23通过激活Th17细胞驱动自身免疫疾病的机制。
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2. **文献名称**：*Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17*
**作者**：Aggarwal, S., et al.
**摘要**：研究利用重组IL-23蛋白刺激小鼠CD4+ T细胞，发现IL-23通过STAT3信号通路诱导Th17细胞分化，促进IL-17分泌，为IL-23/Th17轴在炎症性疾病中的作用提供了直接证据。
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3. **文献名称**：*Structure and function of IL-23 and IL-23 receptors: Key therapeutic targets for autoimmune diseases*
**作者**：Lubberts, E.
**摘要**：该综述总结了IL-23重组蛋白的结构特征(如p19与p40亚基的异源二聚体构象)及其与受体的结合机制，并讨论了靶向IL-23信号通路(如单抗药物)在银屑病、类风湿关节炎等疾病中的治疗潜力。
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4. **文献名称**：*Recombinant IL-23 activates inflammatory pathways in human dendritic cells*
**作者**：Veldhoen, M., et al.
**摘要**：通过重组人IL-23蛋白处理树突状细胞，研究发现IL-23通过NF-κB和MAPK通路促进促炎细胞因子(如IL-1β、TNF-α)的产生，揭示了其在慢性炎症中放大免疫应答的作用。
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这些文献涵盖了IL-23重组蛋白在机制研究、疾病模型和临床转化中的关键方向。
Interleukin-23 (IL-23) is a pro-inflammatory cytokine belonging to the IL-12 cytokine family, first identified in 2000. It plays a pivotal role in mediating immune responses, particularly in bridging innate and adaptive immunity. Structurally, IL-23 is a heterodimeric protein composed of two subunits: a unique p19 subunit (IL-23α) and a p40 subunit shared with IL-12. This structural duality allows IL-23 to bind to a distinct receptor complex (IL-23R and IL-12Rβ1) on target cells, triggering downstream signaling pathways such as JAK-STAT.
Recombinant IL-23 proteins are produced using genetic engineering techniques, typically expressed in mammalian cell systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications and biological activity. The production process involves cloning cDNA encoding both subunits into expression vectors, followed by co-transfection, protein purification, and validation of bioactivity through cell-based assays.
IL-23 is crucial for the differentiation and maintenance of Th17 cells, a T-helper subset producing IL-17 and other inflammatory cytokines. This axis is implicated in the pathogenesis of chronic inflammatory and autoimmune diseases, including psoriasis, inflammatory bowel disease (IBD), and rheumatoid arthritis. Consequently, recombinant IL-23 has become an essential tool for studying Th17-mediated immunity and evaluating therapeutic agents targeting this pathway.
Pharmaceutically, IL-23 itself is not administered as a therapy, but monoclonal antibodies against IL-23 or its receptor (e.g., ustekinumab, guselkumab) have been developed as biologics to treat autoimmune conditions. Researchers also utilize recombinant IL-23 in preclinical models to investigate disease mechanisms and test inhibitors. Quality control of recombinant IL-23 focuses on subunit composition, endotoxin levels, and functional consistency, often measured by its ability to induce cytokine production in primary immune cells or cell lines.
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