| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCN2 |
| Uniprot No | P80188 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-198aa |
| 氨基酸序列 | QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG |
| 预测分子量 | 20.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LCN2(脂质运载蛋白2)重组蛋白的参考文献示例，包含文献名称、作者及摘要内容概括：
1. **《Recombinant LCN2 protein alleviates sepsis-induced acute kidney injury by regulating iron metabolism》**
- 作者：Xu, S. et al. (2020)
- 摘要：该研究通过动物模型证明，重组LCN2蛋白通过调节铁代谢途径减少氧化应激，显著改善败血症诱导的急性肾损伤，并降低炎症因子水平。
2. **《Structural and functional characterization of recombinant human lipocalin-2 in bacterial infection models》**
- 作者：Yang, J. et al. (2018)
- 摘要：作者在大肠杆菌中高效表达重组人LCN2蛋白，发现其通过螯合细菌铁载体抑制病原菌生长，在尿路感染模型中展现出抗菌活性。
3. **《LCN2 promotes breast cancer metastasis through CXCR2 signaling and neutrophil recruitment》**
- 作者：Chakraborty, S. et al. (2016)
- 摘要：研究利用重组LCN2蛋白处理乳腺癌细胞，揭示其通过激活CXCR2信号通路促进中性粒细胞募集，进而增强肿瘤转移能力的分子机制。
4. **《Recombinant LCN2 modulates microglial activation and neuroinflammation in Alzheimer's disease models》**
- 作者：Jiang, H. et al. (2019)
- 摘要：实验表明，外源性重组LCN2蛋白能通过调控小胶质细胞极化和抑制NF-κB通路，减轻阿尔茨海默病模型中的神经炎症和认知损伤。
注：以上文献信息为示例，实际引用时需以具体发表的论文内容为准。建议通过PubMed或Web of Science等平台以关键词“recombinant LCN2 protein”检索最新研究。
**Background of LCN2 Recombinant Protein**
Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a 25-kDa secretory glycoprotein belonging to the lipocalin superfamily. It plays multifaceted roles in innate immunity, iron homeostasis, and inflammatory responses. LCN2 is produced by various cell types, including neutrophils, epithelial cells, and macrophages, and is upregulated during infections, tissue injury, or metabolic stress. Its primary function involves binding to bacterial siderophores (e.g., enterobactin) to limit pathogen growth by sequestering iron, a critical nutrient for microbial proliferation. Additionally, LCN2 modulates cellular iron transport via interactions with mammalian iron carriers, influencing processes like apoptosis and oxidative stress.
Recombinant LCN2 protein is engineered through genetic cloning, typically expressed in *E. coli* or mammalian cell systems, followed by purification to ensure high bioavailability and functional consistency. Its production enables precise study of LCN2's mechanisms in disease models, such as acute kidney injury, cancer progression, and metabolic disorders. For instance, elevated LCN2 levels are linked to chronic inflammation in obesity and diabetes, while its role in cancer remains context-dependent, showing both tumor-promoting and suppressive effects.
Therapeutic potential of recombinant LCN2 is explored for mitigating bacterial infections, promoting tissue repair, or modulating iron dysregulation. However, challenges persist in understanding its dual roles in pathologies and optimizing delivery systems. Research also focuses on its interaction with receptors like 24p3R or megalin, which mediate cellular uptake and signaling. As a biomarker, recombinant LCN2 aids in diagnostic assay development, particularly for renal and inflammatory diseases. Overall, LCN2 recombinant protein serves as a vital tool for deciphering its biological complexity and translational applications.
×
