| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | crtX |
| Uniprot No | P21686 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MSHFAAIAPPFYSHVRALQNLAQELVARGHRVTFIQQYDIKHLIDSETIGFHSVGTDSHPPGALTRVLHLAAHPLGPSMLKLINEMARTTDMLCRELPQAFNDLAVDGVIVDQMEPAGALVAEALGLPFISVACALPLNREPDMPLAVMPFEYGTSDAARERYAASEKIYDWLMRRHDRVIAEHSHRMGLAPRQKLHQCFSPLAQISQLVPELDFPRKALPACFHAVGPLRETHAPSTSSSRYFTSSEKPRIFASLGTLQGHRYGLFKTIVKACEEIDGQLLLAHCGRLTDSQCEELARSRHTQVVDFADQSAALSQAQLAITHGGMNTVLDAINYRTPLLALPLAFDQPGVASRIVYHGIGKRASRFTTSHALARQMRSLLTNVDFQQRMAKIQTALRLAGGTMAAADIIEQVMCTGQPVLSGSGYATAL |
| 预测分子量 | 54.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的3篇关于crtX重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**: Heterologous expression and functional characterization of crtX in Escherichia coli
**作者**: Zhang L., et al.
**摘要**: 本研究成功将Pantoea ananatis的crtX基因克隆至大肠杆菌表达系统，纯化获得重组蛋白。酶活实验证实其具有玉米黄质糖基转移酶活性，最适反应pH为7.5.为类胡萝卜素合成途径研究提供依据。
2. **文献名称**: Structural analysis of CrtX protein from marine bacteria
**作者**: Tanaka K., et al.
**摘要**: 通过X射线晶体学解析了海洋来源Rhodobacter sp.的CrtX蛋白三维结构(分辨率2.1Å)，揭示其底物结合口袋的关键氨基酸残基，为酶分子改造奠定结构基础。
3. **文献名称**: Metabolic engineering of Saccharomyces cerevisiae using crtX for astaxanthin production
**作者**: Chen W., et al.
**摘要**: 将Haematococcus pluvialis的crtX基因导入酵母中，成功实现虾青素前体的糖基化修饰。重组菌株产量提升3.2倍，证明CrtX在微生物合成色素中的关键作用。
建议通过PubMed/Web of Science检索真实文献，使用关键词：
"crtX recombinant protein" "zeaxanthin glucosyltransferase" "carotenoid biosynthesis"
可重点关注Applied Microbiology and Biotechnology、Journal of Biotechnology等期刊文献。
**Background of crtX Recombinant Protein**
The crtX gene encodes a glycosyltransferase enzyme, often associated with bacterial polysaccharide biosynthesis. Originally identified in Gram-negative bacteria, crtX plays a critical role in the synthesis of surface polysaccharides, such as lipopolysaccharides (LPS) or capsular polysaccharides (CPS), which are vital for bacterial virulence, immune evasion, and environmental adaptation. These polysaccharides contribute to pathogenicity by shielding bacteria from host defenses or mediating adhesion to host cells.
Recombinant crtX protein is produced via genetic engineering, where the crtX gene is cloned into an expression vector (e.g., *E. coli* or yeast systems) to enable large-scale protein production. This approach allows for high purity and yield, facilitating structural and functional studies. The recombinant protein retains the enzymatic activity of its native counterpart, catalyzing the transfer of sugar moieties to specific acceptor molecules—a step critical for constructing complex carbohydrate structures.
Research on crtX recombinant protein has broad applications. In biotechnology, it serves as a tool for synthesizing defined oligosaccharides or modifying glycoconjugates. In vaccine development, understanding crtX-mediated polysaccharide synthesis aids in designing glycoconjugate vaccines against pathogenic bacteria. Additionally, crtX is studied to unravel mechanisms of bacterial resistance or to identify inhibitors as potential antimicrobial agents. Its structural characterization (e.g., via X-ray crystallography) further elucidates substrate-binding domains and catalytic mechanisms, guiding rational drug design.
Overall, crtX recombinant protein bridges microbial biochemistry and applied research, offering insights into bacterial pathogenesis while enabling innovations in therapeutic and industrial enzymology.
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