| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CHTD2; FLJ21885; KIAA0733; MAP3K7IP2; TAB2 |
| Entrez GeneID | 23118 |
| clone | 3B5 |
| WB Predicted band size | 80kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TAB2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于TAB2抗体的参考文献及其摘要概括(注：文献为虚构示例，实际文献需通过数据库验证)：
1. **"TAB2 mediates TAK1 activation in IL-1 signaling"**
- **作者**: Takaesu G. et al.
- **摘要**: 研究揭示了TAB2在IL-1信号通路中通过结合K63多聚泛素链激活TAK1的分子机制，使用TAB2特异性抗体验证了其与TAK1复合物的相互作用。
2. **"Structural basis for TAB2 recognition of ubiquitin chains"**
- **作者**: Shim J.H. et al.
- **摘要**: 通过X射线晶体学解析TAB2的C端结构域与K63泛素链的结合模式，实验中使用抗TAB2抗体进行免疫共沉淀，证实其在NF-κB信号中的支架功能。
3. **"TAB2 deficiency impairs angiogenesis via NF-κB pathway"**
- **作者**: Shibuya H. et al.
- **摘要**: 利用TAB2敲除小鼠模型及TAB2抗体检测，发现TAB2缺失导致内皮细胞NF-κB活性下降，进而抑制血管生成相关基因表达。
4. **"Antibody-based profiling of TAB2 in tumor microenvironments"**
- **作者**: Sanjo H. et al.
- **摘要**: 开发高特异性TAB2单克隆抗体，用于免疫组化分析多种癌症组织，揭示TAB2在肿瘤相关巨噬细胞中的高表达及其促炎作用。
建议通过PubMed或Google Scholar以“TAB2 antibody” + “signaling”/“structure”等关键词检索真实文献。
The TAB2 (TGF-beta activated kinase 1/MAP3K7 binding protein 2) antibody is a tool used to study the TAB2 protein, a key adaptor molecule in intracellular signaling pathways. TAB2 facilitates signal transduction by linking upstream regulators like TRAF6 to downstream kinases such as TAK1 (MAP3K7), particularly in the NF-κB and MAPK pathways. These pathways are central to innate immunity, inflammation, and stress responses, making TAB2 critical in processes like cytokine production, cell survival, and apoptosis.
TAB2 dysfunction has been implicated in diseases including cancers, autoimmune disorders, and cardiovascular conditions. For example, chromosomal deletions or mutations in TAB2 are associated with congenital heart defects and Noonan syndrome-like syndromes. Its role in modulating inflammatory signals also links it to conditions like rheumatoid arthritis and metabolic disorders.
Antibodies targeting TAB2 enable researchers to detect protein expression, localization, and interaction partners via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specific TAB2 antibodies help investigate post-translational modifications (e.g., ubiquitination) or subcellular trafficking under stimuli such as TNF-α, IL-1β, or pathogen-associated molecules. Commercial TAB2 antibodies are typically validated for cross-reactivity, epitope specificity (e.g., C-terminal or N-terminal regions), and compatibility with human, mouse, or rat samples. These reagents are essential for dissecting TAB2's context-dependent roles in health and disease.
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