| 纯度 | >90%SDS-PAGE. |
| 种属 | Drosophila |
| 靶点 | RpII215 |
| Uniprot No | P08775 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1579-1881aa |
| 氨基酸序列 | YSPTSPNYTASSPGGASPNYSPSSPNYSPTSPLYASPRYASTTPNFNPQSTGYSPSSSGYSPTSPVYSPTVQFQSSPSFAGSGSNIYSPGNAYSPSSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTPSYSPTSPNYSASPQYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGSPGSPQYTPGSPQYSPASPKYSPTSPLYSPSSPQHSPSNQYSPTGSTYSATSPRYSPNMSIYSPSSTKYSPTSPTYTPTARNYSPTSPMYSPTAPSHYSPTSPAYSPSSPT |
| 预测分子量 | 33.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RpII215(RNA聚合酶II最大亚基)重组蛋白的示例参考文献(内容为模拟示例，建议通过学术数据库获取真实文献)：
1. **文献名称**：*Expression and purification of recombinant RpII215 for structural studies*
**作者**：Smith J, et al.
**摘要**：该研究成功在大肠杆菌中表达了RpII215重组蛋白，并通过亲和层析纯化获得高纯度蛋白。作者利用该蛋白进行了初步的晶体学分析，为解析RNA聚合酶II的全结构奠定了基础。
2. **文献名称**：*Functional analysis of RpII215 in transcription elongation using recombinant protein assays*
**作者**：Lee S, Brown T.
**摘要**：通过体外转录实验，验证了重组RpII215蛋白在转录延伸过程中的关键作用，并发现其CTD(C端结构域)磷酸化状态直接影响mRNA加工效率。
3. **文献名称**：*Disease-associated mutations in RpII215 disrupt recombinant protein stability*
**作者**：Zhang Y, et al.
**摘要**：研究了癌症相关RpII215突变体对重组蛋白稳定性的影响，发现特定突变导致蛋白降解加速，揭示了其与转录失调疾病的潜在关联。
4. **文献名称**：*Cryo-EM structure of recombinant RpII215 complex reveals TFIIH interaction*
**作者**：Martinez R, et al.
**摘要**：利用冷冻电镜解析了RpII215重组蛋白与转录因子TFIIH的复合物结构，阐明了二者在启动子逃逸阶段的协同机制。
**注意**：以上内容为示例，实际文献需通过PubMed、Web of Science等平台检索关键词(如“RpII215 recombinant”、“POLR2A expression”)获取。
**Background of RpII215 Recombinant Protein**
RpII215. also known as RNA polymerase II subunit B1. is a critical component of the RNA polymerase II (Pol II) enzyme complex, which plays a central role in eukaryotic transcription. As the largest subunit of Pol II, RpII215 (ortholog of human POLR2A) forms the catalytic core responsible for synthesizing messenger RNA (mRNA) during gene expression. It contains a C-terminal domain (CTD) composed of heptapeptide repeats that undergo dynamic phosphorylation, a process essential for regulating transcription initiation, elongation, and termination.
The recombinant RpII215 protein is engineered using expression systems (e.g., *E. coli*, insect, or mammalian cells*) to enable precise study of its structure and function. By eliminating confounding cellular factors, recombinant forms allow researchers to dissect mechanistic details of transcription, such as CTD modification interactions with transcription factors (e.g., TFIIH) or the role of mutations in diseases like cancer and neurodegeneration.
Studies leveraging RpII215 recombinant proteins have advanced understanding of Pol II’s role in gene regulation, including its coupling with mRNA capping, splicing, and chromatin remodeling. Additionally, it serves as a tool for drug screening, particularly targeting transcription dysregulation in pathologies. Structural analyses (e.g., cryo-EM) using recombinant RpII215 have resolved conformational states during transcription, offering insights into therapeutic intervention strategies.
Overall, RpII215 recombinant protein remains a pivotal resource for unraveling transcriptional mechanisms and their implications in health and disease.
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