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Recombinant Human Nos3 protein

  • 中文名: 一氧化氮合酶3(Nos3)重组蛋白
  • 别    名: Nos3;Nitric oxide synthase 3
货号: PA2000-3687
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点Nos3
Uniprot No P29474
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-1203aa
氨基酸序列MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP
预测分子量133,2 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于Nos3(eNOS)重组蛋白研究的示例参考文献,格式为文献名称、作者及摘要概括:

1. **"Cloning and expression of endothelial nitric oxide synthase"**

*Marsden, P. A., et al. (1992)*

摘要:首次报道了人内皮型一氧化氮合酶(eNOS)的基因克隆,并在哺乳动物细胞中成功表达重组蛋白,证实其催化生成一氧化氮的功能,为后续研究eNOS的生物学作用奠定基础。

2. **"Functional reconstitution of endothelial nitric oxide synthase reveals the importance of tetrahydrobiopterin in dimer formation"**

*List, B. M., et al. (1997)*

摘要:通过重组eNOS蛋白的功能重建实验,证明四氢生物蝶呤(BH4)对酶的二聚化及活性至关重要,揭示了辅因子在eNOS催化机制中的关键作用。

3. **"Baculovirus-mediated expression of recombinant endothelial nitric oxide synthase: Characterization of the enzyme activity and post-translational modifications"**

*McMillan, K., et al. (1992)*

摘要:利用杆状病毒系统在昆虫细胞中高效表达重组eNOS,分析其酶动力学特性及磷酸化等翻译后修饰,为大规模制备活性eNOS提供方法。

4. **"Targeted disruption of the endothelial nitric oxide synthase gene in mice: Insights into cardiovascular phenotypes"**

*Shesely, E. G., et al. (1996)*

摘要:通过基因敲除技术构建eNOS缺陷小鼠模型,结合重组蛋白功能研究,阐明eNOS在血管稳态和血压调控中的生理意义。

**说明**:以上文献为示例性质,作者和标题参考了真实研究方向的代表性工作,具体引用时建议通过PubMed或Google Scholar核实最新及完整文献信息。

背景信息

**Background of Nos3 Recombinant Protein**

Nos3. also known as endothelial nitric oxide synthase (eNOS), is a critical enzyme encoded by the *NOS3* gene in humans. It catalyzes the production of nitric oxide (NO) from L-arginine, a signaling molecule essential for regulating vascular tone, blood flow, and endothelial function. NO derived from eNOS plays a pivotal role in maintaining cardiovascular homeostasis, including inhibition of platelet aggregation, smooth muscle relaxation, and modulation of inflammatory responses. Dysregulation of eNOS activity or expression is linked to cardiovascular pathologies such as hypertension, atherosclerosis, and endothelial dysfunction.

Recombinant Nos3 protein is engineered using molecular cloning techniques to enable large-scale production for research and therapeutic applications. The protein is typically expressed in heterologous systems like *E. coli*, yeast, or mammalian cell lines (e.g., HEK293 or CHO cells) to ensure proper folding and post-translational modifications, which are crucial for its enzymatic activity. The recombinant form often includes affinity tags (e.g., His-tag) for simplified purification. Structurally, Nos3 is a homodimer with binding domains for cofactors such as NADPH, flavin mononucleotide (FMN), and calmodulin, which regulate its activity in response to calcium levels.

Studies utilizing recombinant Nos3 focus on elucidating its mechanistic role in vascular biology, drug screening for cardiovascular diseases, and exploring mutations associated with impaired NO signaling. Its application extends to developing therapies targeting endothelial dysfunction or enhancing NO bioavailability. Challenges in production include maintaining stability and activity due to its complex regulation, necessitating optimized expression systems. Overall, recombinant Nos3 serves as a vital tool for advancing cardiovascular research and translational medicine.

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