Recombinant E.coli rpsM protein

中文名： 核糖体小亚基蛋白uS13(rpsM)重组蛋白
别名： rpsM；Small ribosomal subunit protein uS13

货号: PA2000-3631

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	E.coli
靶点	rpsM
Uniprot No	P0A7S9
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	2-118aa
氨基酸序列	ARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVKISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKPIKK
预测分子量	40.0 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于rpsM重组蛋白的模拟参考文献示例，供参考：

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1. **文献名称**: *Cloning and Expression of Recombinant rpsM in Escherichia coli for Structural Studies*

**作者**: Zhang L, et al.

**摘要**: 本研究成功克隆了肺炎链球菌的rpsM基因，并利用大肠杆菌表达系统实现重组蛋白的可溶性表达。通过亲和层析纯化获得高纯度蛋白，并采用圆二色光谱分析其二级结构，为后续功能研究奠定基础。

2. **文献名称**: *Functional Characterization of rpsM in Bacterial Ribosome Assembly*

**作者**: Tanaka K, et al.

**摘要**: 通过敲除和互补实验，验证了rpsM在细菌核糖体30S亚基组装中的关键作用。重组rpsM蛋白的体外重构实验表明，其缺失导致核糖体功能异常，影响蛋白质翻译效率。

3. **文献名称**: *Recombinant rpsM as a Potential Vaccine Candidate Against Mycobacterium tuberculosis*

**作者**: Singh R, et al.

**摘要**: 评估结核分枝杆菌rpsM重组蛋白的免疫原性，发现其在小鼠模型中能诱导Th1型免疫反应，显著提高对结核杆菌攻击的保护效力，提示其作为亚单位疫苗的潜力。

4. **文献名称**: *Crystallization and X-ray Analysis of rpsM from Bacillus subtilis*

**作者**: Müller J, et al.

**摘要**: 首次报道枯草芽孢杆菌rpsM重组蛋白的晶体结构解析，揭示其与16S rRNA结合的分子机制，为抗生素靶点设计提供结构依据。

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注：以上文献为示例性质，实际引用时需以真实发表的论文为准。建议通过PubMed、Google Scholar等平台检索关键词(如"rpsM recombinant protein")获取具体文献。

背景信息

**Background of rpsM Recombinant Protein**

The *rpsM* gene encodes ribosomal protein S13. a critical component of the 30S subunit in prokaryotic ribosomes. As part of the translation machinery, S13 plays a structural and functional role in protein synthesis, contributing to ribosome assembly, mRNA decoding, and tRNA binding. Its conserved sequence across bacterial species underscores its essentiality in cellular viability and growth.

Recombinant rpsM protein is produced through genetic engineering, where the *rpsM* gene is cloned into expression vectors and expressed in heterologous systems like *Escherichia coli*. This allows large-scale production of the protein with high purity and solubility, often facilitated by affinity tags (e.g., His-tags). Recombinant technology enables precise study of S13’s biochemical properties, interactions, and structural dynamics, which are challenging to explore in native ribosomes due to their complexity.

Research on rpsM recombinant protein holds significance in multiple areas. Structurally, it aids in elucidating ribosome assembly mechanisms and the role of S13 in maintaining translational fidelity. Functionally, it serves as a tool to investigate antibiotic targeting, as some antimicrobials bind to ribosomal proteins to disrupt bacterial protein synthesis. Additionally, recombinant S13 is used to study protein-RNA interactions and validate genetic knockouts in synthetic biology.

The development of rpsM recombinant protein also supports drug discovery, particularly in designing inhibitors against pathogenic bacteria. By understanding S13’s role in antibiotic resistance or susceptibility, researchers can identify novel therapeutic strategies. Overall, rpsM recombinant protein is a vital resource for advancing molecular microbiology, structural biology, and antimicrobial development.