Recombinant E.coli isaB protein

中文名： 金黄色葡萄球菌免疫显性葡萄球菌抗原B(isaB)重组蛋白
别名： isaB；Immunodominant staphylococcal antigen B

货号: PA2000-3474

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	E.coli
靶点	isaB
Uniprot No	Q9LAB5
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	37-175aa
氨基酸序列	AITPYYTYNGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEASQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNRVVEVTVGHTPYKTSK
预测分子量	20.8 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下为虚构示例文献，供参考写作框架：

1. **文献名称**: "Role of recombinant IsaB in Staphylococcus aureus immune evasion"

**作者**: Müller et al.

**摘要**: 研究证实isaB重组蛋白通过抑制中性粒细胞胞外陷阱(NETs)的形成，增强金黄色葡萄球菌对宿主免疫系统的抵抗能力，揭示其作为潜在治疗靶点的可能性。

2. **文献名称**: "Expression and functional analysis of IsaB in S. aureus cell wall remodeling"

**作者**: Zhang & Vandenesch

**摘要**: 通过重组IsaB蛋白的体外实验，发现其参与细菌细胞壁的完整性维持，并影响溶菌酶介导的细胞裂解过程，为抗生素开发提供新方向。

3. **文献名称**: "Immunogenicity evaluation of recombinant IsaB as a vaccine candidate"

**作者**: Silva et al.

**摘要**: 在小鼠模型中评估重组IsaB蛋白的免疫原性，显示其可诱导保护性抗体反应，降低金黄色葡萄球菌感染后的细菌负荷，提示疫苗应用潜力。

4. **文献名称**: "Structural characterization of IsaB protein in MRSA isolates"

**作者**: Lee et al.

**摘要**: 利用X射线晶体学解析isaB重组蛋白的三维结构，发现其保守结构域与宿主补体蛋白的相互作用位点，为设计抑制剂奠定基础。

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注：以上内容为示例性虚构，实际文献需通过PubMed/Web of Science等平台检索关键词 "IsaB recombinant protein Staphylococcus aureus" 获取。

背景信息

**Background of IsaB Recombinant Protein**

IsaB (immunodominant staphylococcal antigen B) is a cell wall-associated protein originally identified in *Staphylococcus aureus*, a major bacterial pathogen responsible for diverse infections, ranging from skin abscesses to life-threatening sepsis. The *isaB* gene is part of the *isaA* (immunodominant staphylococcal antigen A) operon, which is regulated by the *sigB* stress-response sigma factor. IsaB is expressed during bacterial stress conditions, such as nutrient limitation or host immune challenges, suggesting a role in *S. aureus* adaptation and survival within hostile environments.

Studies indicate that IsaB contributes to immune evasion by interacting with host components. It exhibits structural similarities to other staphylococcal surface proteins involved in adhesion, biofilm formation, or modulation of host defenses. While its precise mechanistic role remains under investigation, IsaB has been implicated in reducing bacterial susceptibility to phagocytosis, potentially by masking pathogen-associated molecular patterns (PAMPs) or interfering with opsonization.

Recombinant IsaB protein is produced via heterologous expression systems (e.g., *E. coli*) for research and therapeutic applications. Its production enables the study of *S. aureus* pathogenesis, host-pathogen interactions, and immune responses. Recombinant IsaB is also explored as a diagnostic antigen or vaccine candidate due to its immunogenicity. Antibodies against IsaB have been detected in patients with *S. aureus* infections, highlighting its relevance as a target for immune-based strategies.

Overall, IsaB recombinant protein serves as a critical tool for understanding staphylococcal virulence and advancing novel antimicrobial interventions.