| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NMNAT1 |
| Uniprot No | Q9HAN9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-279aa |
| 氨基酸序列 | MENSEKTEVVLLACGSFNPITNMHLRLFELAKDYMNGTGRYTVVKGIISPVGDAYKKKGLIPAYHRVIMAELATKNSKWVEVDTWESLQKEWKETLKVLRHHQEKLEASDCDHQQNSPTLERPGRKRKWTETQDSSQKKSLEPKTKAVPKVKLLCGADLLESFAVPNLWKSEDITQIVANYGLICVTRAGNDAQKFIYESDVLWKHRSNIHVVNEWIANDISSTKIRRALRRGQSIRYLVPDLVQEYIEKHNLYSSESEDRNAGVILAPLQRNTAEAKT |
| 预测分子量 | 58.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NMNAT1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural and functional analysis of human NMNAT1 reveals a key regulatory role in NAD biosynthesis*
**作者**：Ali YO, et al.
**摘要**：本研究通过重组表达人源NMNAT1蛋白，解析其晶体结构，并验证其催化NAD+合成的关键酶活性，揭示了其在神经元保护中的作用机制。
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2. **文献名称**：*Recombinant NMNAT1 attenuates axonal degeneration in a cellular model of Parkinson's disease*
**作者**：Jayabalan B, et al.
**摘要**：利用重组NMNAT1蛋白处理帕金森病细胞模型，发现其通过增强NAD+水平和抑制SARM1通路，显著延缓轴突变性，提示其治疗神经退行性疾病的潜力。
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3. **文献名称**：*High-yield production of NMNAT1 in E. coli and its enzymatic characterization for drug screening*
**作者**：Zhou X, et al.
**摘要**：报道了在大肠杆菌中高效表达和纯化重组NMNAT1蛋白的方法，并对其酶动力学参数进行分析，为基于NAD+代谢的药物开发提供技术基础。
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(注：以上文献为示例，实际引用时需核对真实来源及准确性。)
**Background of NMNAT1 Recombinant Protein**
NMNAT1 (Nicotinamide Nucleotide Adenylyltransferase 1) is a ubiquitously expressed enzyme that catalyzes the final step in the biosynthesis of nicotinamide adenine dinucleotide (NAD⁺), a critical coenzyme involved in cellular energy metabolism, DNA repair, and signaling pathways. As one of three NMNAT isoforms in humans, NMNAT1 is distinct in its nuclear localization and role in maintaining NAD⁺ homeostasis, particularly in metabolically active or stressed cells. Dysregulation of NMNAT1 has been linked to neurodegenerative disorders, such as Alzheimer’s and Parkinson’s diseases, as well as retinal degeneration, highlighting its importance in neuronal survival and function.
Recombinant NMNAT1 protein is engineered using genetic cloning techniques, typically expressed in *E. coli* or mammalian cell systems*,* to ensure high purity and functional activity. Its production enables researchers to study the enzyme’s structure, substrate specificity, and interactions with other proteins or small molecules. Notably, NMNAT1 exhibits neuroprotective properties independent of its enzymatic activity, suggesting additional chaperone-like roles in protein quality control. This dual functionality makes it a compelling therapeutic target for conditions involving axonal degeneration or mitochondrial dysfunction.
In research, NMNAT1 recombinant protein is utilized to investigate mechanisms of NAD⁺ depletion in aging, metabolic disorders, and neurodegeneration. It also serves as a tool for drug discovery, screening compounds that modulate NAD⁺ synthesis or enhance cellular stress resilience. Recent studies emphasize its potential in delaying age-related pathologies by boosting NAD⁺ levels, offering avenues for developing therapies against diseases driven by metabolic decline.
Overall, NMNAT1 recombinant protein bridges fundamental research and translational applications, providing insights into cellular health and strategies to combat NAD⁺-related disorders.
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