| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Mup2 |
| Uniprot No | P11589 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-180aa |
| 氨基酸序列 | EEASSTGRNFNVEKINGEWHTIILASDKREKIEDNGNFRLFLEQIHVLEKSLVLKFHTVRDEECSELSMVADKTEKAGEYSVTYDGFNTFTIPKTDYDNFLMAHLINEKDGETFQLMGLYGREPDLSSDIKERFAKLCEEHGILRENIIDLSNANRCLQARE |
| 预测分子量 | 20.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MUP2重组蛋白的3篇文献概览(内容为虚构示例，供参考)：
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1. **文献名称**: *Expression and Characterization of Recombinant MUP2 in Escherichia coli*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组MUP2蛋白，通过质谱和圆二色光谱验证其正确折叠。实验表明，重组MUP2能够特异性结合信息素类似物，提示其在哺乳动物化学通讯中的潜在作用。
2. **文献名称**: *Structural Insights into MUP2-Ligand Interactions by X-ray Crystallography*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了重组MUP2与棕榈酸酯复合物的三维结构，揭示了其疏水结合口袋的关键氨基酸残基。该研究为理解MUP家族蛋白的配体选择性提供了分子基础。
3. **文献名称**: *Functional Role of Recombinant MUP2 in Mouse Social Behavior*
**作者**: Tanaka K, et al.
**摘要**: 利用重组MUP2进行的行为学实验表明，该蛋白通过尿液释放影响小鼠的领地标记行为。研究还发现MUP2缺失突变体的社会互动能力显著降低，证实了其在社交信号传递中的重要性。
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**注**：以上内容为示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索关键词“MUP2 recombinant protein”或“Major urinary protein 2 expression”获取。如需真实文献，建议提供具体研究背景(如物种或功能方向)。
**Background of Mup2 Recombinant Protein**
Murine major urinary protein 2 (Mup2) belongs to the lipocalin superfamily, a group of small extracellular proteins involved in binding and transporting hydrophobic molecules. MUPs are primarily synthesized in the liver and excreted in urine, playing critical roles in chemical communication, particularly in rodents. Mup2 is notable for its high affinity for pheromones and volatile organic compounds, which mediate social and reproductive behaviors in mice. Its ability to stabilize and slowly release these signaling molecules makes it essential for territorial marking and mate attraction.
Recombinant Mup2 protein is engineered using biotechnological platforms, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The recombinant form retains the native protein’s structural and functional properties, including ligand-binding capabilities. Researchers leverage recombinant Mup2 to study olfactory signaling mechanisms, ligand-protein interactions, and evolutionary adaptations in chemical communication.
Beyond behavioral studies, Mup2 has biomedical relevance. Emerging evidence links MUPs to metabolic regulation, with Mup2 implicated in glucose homeostasis and lipid metabolism. Its recombinant version facilitates *in vitro* and *in vivo* studies exploring these pathways, offering insights into metabolic disorders such as diabetes and obesity. Additionally, recombinant Mup2 serves as a tool for developing biosensors or drug-delivery systems due to its stable binding pockets.
The production of recombinant Mup2 also addresses challenges in sourcing native proteins from biological samples, ensuring scalability, consistency, and ethical compliance. Its applications span ecology, neuroscience, and molecular medicine, underscoring its versatility as a model protein in interdisciplinary research.
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