Recombinant Human EHF protein

中文名： Ets同源物因子(EHF)重组蛋白
别名： EHF；ESE3；ESE3B；ESEJ;ETS homologous factor

货号: PA1000-986DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	EHF
Uniprot No	Q9NZC4
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-300aa
氨基酸序列	MGSSHHHHHH SSGLVPRGSH MGSMILEGGG VMNLNPGNNL LHQPPAWTDS YSTCNVSSGF FGGQWHEIHP QYWTKYQVWE WLQHLLDTNQ LDANCIPFQE FDINGEHLCS MSLQEFTRAA GTAGQLLYSN LQHLKWNGQC SSDLFQSTHN VIVKTEQTEP SIMNTWKDEN YLYDTNYGST VDLLDSKTFC RAQISMTTTS HLPVAESPDM KKEQDPPAKC HTKKHNPRGT HLWEFIRDIL LNPDKNPGLI KWEDRSEGVF RFLKSEAVAQ LWGKKKNNSS MTYEKLSRAM RYYYKREILE RVDGRRLVYK FGKNARGWRE NEN
预测分子量	37 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇关于EHF(Epithelium-specific Ets transcription factor)重组蛋白的参考文献摘要，供参考：

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1. **文献名称**: *"Recombinant EHF protein modulates epithelial barrier function through claudin-1 regulation"*

**作者**: Smith J, et al.

**摘要**: 本研究通过大肠杆菌表达系统制备重组EHF蛋白，发现其通过直接结合claudin-1基因启动子增强肠上皮细胞屏障功能，为炎症性肠病治疗提供潜在靶点。

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2. **文献名称**: *"Structural and functional analysis of EHF in prostate cancer progression"*

**作者**: Lee H, et al.

**摘要**: 利用哺乳动物细胞系表达纯化的EHF重组蛋白，揭示其通过抑制EGFR信号通路抑制前列腺癌细胞侵袭，提示EHF作为抑癌因子的分子机制。

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3. **文献名称**: *"EHF recombinant protein attenuates airway inflammation in a murine asthma model"*

**作者**: Wang Y, et al.

**摘要**: 通过昆虫细胞表达系统获得高活性EHF重组蛋白，动物实验表明其通过下调IL-33和Th2细胞因子减轻哮喘模型气道炎症，为呼吸系统疾病治疗提供新策略。

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(注：以上文献信息为模拟概括，实际引用请核对真实数据库如PubMed或Web of Science。)

背景信息

EHF (ETS Homologous Factor), also known as SAM-pointed domain-containing ETS transcription factor (SPDEF), is a member of the ETS family of transcription factors that play critical roles in regulating gene expression during development, immunity, and cellular differentiation. Discovered in the late 1990s, EHF is primarily expressed in epithelial tissues, including the respiratory, gastrointestinal, and urogenital tracts, where it modulates cell proliferation, barrier function, and immune responses. Its structure features a conserved ETS DNA-binding domain and a unique N-terminal transactivation domain, enabling sequence-specific gene regulation.

Research has linked EHF to diverse physiological and pathological processes. It acts as a tumor suppressor in certain cancers (e.g., prostate, colorectal) by inhibiting oncogenic pathways, while paradoxically promoting metastasis in others. In respiratory diseases like asthma and chronic obstructive pulmonary disease (COPD), EHF regulates mucus production and inflammation. Its role in maintaining epithelial integrity also connects it to inflammatory bowel disease (IBD) and cystic fibrosis.

Recombinant EHF proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to study its molecular interactions or therapeutic potential. These purified proteins retain functional domains for binding DNA or interacting with co-regulators like AP-1 or NF-κB. Applications include in vitro assays to map target genes, drug screening for EHF-modulating compounds, and mechanistic studies of epithelial disorders. Recent advances in structural biology and gene-editing tools have accelerated research into EHF's context-dependent functions, highlighting its dual roles in health and disease. However, challenges remain in understanding its tissue-specific regulation and therapeutic targeting due to complex signaling crosstalk.