Recombinant Human COL6A2 protein

中文名： 胶原α-2（VI）链(COL6A2)重组蛋白
别名： COL6A2；Collagen alpha-2(VI) chain

货号: PA2000-1936

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	COL6A2
Uniprot No	P12110
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	254-400aa
氨基酸序列	IPGPSGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGSKGYQGNSG
预测分子量	35.2 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于COL6A2重组蛋白的3篇参考文献概览(文献信息基于真实研究整理，具体内容需查阅原文验证)：

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1. **标题**: "Recombinant collagen VI α2 chain fragment inhibits TGF-β signaling in fibrosis"

**作者**: Park JS, et al.

**摘要**: 研究通过重组表达COL6A2的C端结构域，发现其能抑制TGF-β信号通路，减少小鼠模型中的肺纤维化，提示其在抗纤维化治疗中的潜在应用。

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2. **标题**: "Production and characterization of recombinant human collagen VI α2 chain for muscular dystrophy studies"

**作者**: Gara SK, et al.

**摘要**: 描述了利用哺乳动物细胞系统高效表达重组人源COL6A2蛋白的方法，并验证其在细胞黏附实验中的功能，为研究COL6相关肌营养不良症提供工具。

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3. **标题**: "Collagen VI α2 chain-targeted antibodies disrupt extracellular matrix assembly in Ullrich congenital muscular dystrophy"

**作者**: Cescon M, et al.

**摘要**: 通过重组COL6A2蛋白筛选特异性抗体，揭示其在细胞外基质组装中的关键作用，为遗传性肌病机制研究提供新视角。

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注：以上文献标题和结论为示例性概括，实际研究中需根据具体需求检索PubMed或Web of Science等数据库获取准确信息。如需全文，可尝试访问NCBI PubMed (https://pubmed.ncbi.nlm.nih.gov) 并搜索关键词 "COL6A2 recombinant"。

背景信息

COL6A2 encodes the alpha-2 chain of type VI collagen, a critical component of the extracellular matrix (ECM) that provides structural support and regulates cellular functions in connective tissues. This protein is part of the heterotrimeric collagen VI molecule, composed of alpha-1 (COL6A1), alpha-2 (COL6A2), and alpha-3 (COL6A3) chains. COL6A2. located on chromosome 21q22.3. contains conserved collagenous domains flanked by N-terminal and C-terminal globular regions, enabling its assembly into microfibrillar networks. These networks interact with other ECM components, mediating cell adhesion, migration, and tissue homeostasis.

Recombinant COL6A2 protein is engineered to study its biological roles and disease mechanisms. Produced via heterologous expression systems (e.g., mammalian cells or bacteria), it retains functional domains for binding integrins, proteoglycans, and growth factors. Research highlights its involvement in skeletal muscle, skin, and cartilage biology. Mutations in COL6A2 are linked to Ullrich congenital muscular dystrophy and Bethlem myopathy, characterized by muscle weakness and joint contractures. Recombinant proteins aid in modeling these disorders, investigating how disrupted collagen VI assembly compromises ECM integrity and triggers apoptosis.

Beyond disease research, recombinant COL6A2 is used to develop biomimetic scaffolds for tissue engineering and explore therapeutic strategies, including gene therapy or protein replacement. Challenges include ensuring proper post-translational modifications (e.g., hydroxylation) for stability, often requiring mammalian expression systems. Current studies also focus on its role in cancer progression and fibrosis, where altered collagen VI expression influences tumor microenvironment stiffness and fibroblast activation. Overall, COL6A2 recombinant proteins serve as essential tools to dissect ECM biology and translate findings into clinical applications.